View source for 6kbw


==Crystal structure of Tmm from Myroides profundi D25==
<StructureSection load='6kbw' size='340' side='right'caption='[[6kbw]], [[Resolution|resolution]] 1.69&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6kbw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Myroides_profundi Myroides profundi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KBW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6KBW FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.686&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=NAP:NADP+NICOTINAMIDE-ADENINE-DINUCLEOTIDE+PHOSPHATE'>NAP</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6kbw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kbw OCA], [https://pdbe.org/6kbw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6kbw RCSB], [https://www.ebi.ac.uk/pdbsum/6kbw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6kbw ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A0B5RNJ4_9FLAO A0A0B5RNJ4_9FLAO] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
High hydrostatic pressure (HHP) is a characteristic environmental factor of the deep ocean. However, it remains unclear how piezotolerant bacteria adapt to HHP. Here, we identify a two-step metabolic pathway to cope with HHP stress in a piezotolerant bacterium. Myroides profundi D25(T), obtained from a deep-sea sediment, can take up trimethylamine (TMA) through a previously unidentified TMA transporter, TmaT, and oxidize intracellular TMA into trimethylamine N-oxide (TMAO) by a TMA monooxygenase, MpTmm. The produced TMAO is accumulated in the cell, functioning as a piezolyte, improving both growth and survival at HHP. The function of the TmaT-MpTmm pathway was further confirmed by introducing it into Escherichia coli and Bacillus subtilis Encoded TmaT-like and MpTmm-like sequences extensively exist in marine metagenomes, and other marine Bacteroidetes bacteria containing genes encoding TmaT-like and MpTmm-like proteins also have improved HHP tolerance in the presence of TMA, implying the universality of this HHP tolerance strategy in marine Bacteroidetes.

Oxidation of trimethylamine to trimethylamine N-oxide facilitates high hydrostatic pressure tolerance in a generalist bacterial lineage.,Qin QL, Wang ZB, Su HN, Chen XL, Miao J, Wang XJ, Li CY, Zhang XY, Li PY, Wang M, Fang J, Lidbury I, Zhang W, Zhang XH, Yang GP, Chen Y, Zhang YZ Sci Adv. 2021 Mar 26;7(13). pii: 7/13/eabf9941. doi: 10.1126/sciadv.abf9941., Print 2021 Mar. PMID:33771875<ref>PMID:33771875</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6kbw" style="background-color:#fffaf0;"></div>

==See Also==
*[[Monooxygenase 3D structures|Monooxygenase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Myroides profundi]]
[[Category: Li CY]]
[[Category: Zhang YZ]]