View source for 6kcx


==Crystal structure of citrate complex of alpha-glucuronidase (TM0752)from Thermotoga maritima==
<StructureSection load='6kcx' size='340' side='right'caption='[[6kcx]], [[Resolution|resolution]] 1.93&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6kcx]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermotoga_maritima_MSB8 Thermotoga maritima MSB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KCX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6KCX FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.933&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=IMD:IMIDAZOLE'>IMD</scene>, <scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6kcx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kcx OCA], [https://pdbe.org/6kcx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6kcx RCSB], [https://www.ebi.ac.uk/pdbsum/6kcx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6kcx ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q9WZL1_THEMA Q9WZL1_THEMA] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Glycosyl hydrolases belonging to the family 4 (GH4) use a unique redox-based NAD(+)-dependent reaction mechanism involving anionic intermediates and requires a divalent metal ion and reducing conditions for catalytic activity. These enzymes display wide specificity and selectivity for their substrates. However, the structural basis of substrate binding, recognition and specificity remains poorly studied. Here, we report the crystal structure of Thermotoga maritima TmAgu4B, a GH4 alpha-glucuronidase, in complex with Co(2+) and citrate. Analysis of GH4 structures show that the metal ion is present in a conserved octahedral coordination with conserved side chain atoms, the ligand atoms and an invariant water molecule. The data provides the first structural evidence for a metal-activated hydroxide ion that acts as the general base to deprotonate the C3-hydroxyl group of the glycone, a rate-limiting step in the mechanism. Furthermore, the citrate binding mode in the active site is analogous to a bound glucuronide substrate and provides insights into the mode of substrate interaction with the metal ion, the active site residues and, the structural basis of substrate recognition in a GH4 alpha-glucuronidase.

Structure of an alpha-glucuronidase in complex with Co(2+) and citrate provides insights into the mechanism and substrate recognition in the family 4 glycosyl hydrolases.,Mohapatra SB, Manoj N Biochem Biophys Res Commun. 2019 Oct 15;518(2):197-203. doi:, 10.1016/j.bbrc.2019.08.030. Epub 2019 Aug 10. PMID:31409483<ref>PMID:31409483</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6kcx" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Thermotoga maritima MSB8]]
[[Category: Manoj N]]
[[Category: Mohapatra BS]]