View source for 6kmy


==Structure of single disulfide peptide Czon1107-P5A==
<StructureSection load='6kmy' size='340' side='right'caption='[[6kmy]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6kmy]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Conus_zonatus Conus zonatus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KMY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6KMY FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 28 models</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6kmy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kmy OCA], [https://pdbe.org/6kmy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6kmy RCSB], [https://www.ebi.ac.uk/pdbsum/6kmy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6kmy ProSAT]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
Conopeptides are neurotoxic peptides in the venom of marine cone snails and have broad therapeutic potential for managing pain and other conditions. Here, we identified the single-disulfide peptides Czon1107 and Cca1669 from the venoms of Conus zonatus and Conus caracteristicus, respectively. We observed that Czon1107 strongly inhibits the human alpha3beta4 (IC50 15.7 +/- 3.0 muM) and alpha7 (IC50 77.1 +/- 0.05 muM) nicotinic acetylcholine receptor (nAChR) subtypes, but the activity of Cca1669 remains to be identified. Czon1107 acted at a site distinct from the orthosteric receptor site. Solution NMR experiments revealed that Czon1107 exists in equilibrium between conformational states that are the result of a key Ser(4)-Pro(5) cis-trans isomerization. Moreover, we found that the X-Pro amide bonds in the inter-cysteine loop are rigidly constrained to cis conformations. Structure-activity experiments of Czon1107 and its variants at positions P5 and P7 revealed that the conformation around the X-Pro bonds (cis-trans) plays an important role in receptor subtype selectivity. The cis conformation at the Cys(6)-Pro(7) peptide bond was essential for alpha3beta4 nAChR subtype allosteric selectivity. In summary, we have identified an unique single disulfide conopeptide with a non-competitive, potentially allosteric inhibitory mechanism at the nAChRs. The small size and rigidity of the Czon1107 peptide could provide a scaffold for rational drug design strategies for allosteric nAChR modulation. This new paradigm in the "conotoxinomic" structure-function space provides an impetus to screen venom from other Conus species for similar, short bioactive peptides that allosterically modulate ligand-gated receptor function.

Structure and allosteric activity of a single disulfide conopeptide from Conus zonatus at human alpha3beta4 and alpha7 nicotinic acetylcholine receptors.,Mohan MK, Abraham N, R P R, Jayaseelan BF, Ragnarsson L, Lewis RJ, Sarma SP J Biol Chem. 2020 Mar 31. pii: RA119.012098. doi: 10.1074/jbc.RA119.012098. PMID:32234761<ref>PMID:32234761</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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== References ==
<references/>
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</StructureSection>
[[Category: Conus zonatus]]
[[Category: Large Structures]]
[[Category: Madhan Kumar M]]
[[Category: Sarma SP]]