View source for 6kod


==Cu(II) complex of HOCl-induced flavoprotein disulfide reductase RclA C43S mutant from Escherichia coli==
<StructureSection load='6kod' size='340' side='right'caption='[[6kod]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6kod]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_BL21(DE3) Escherichia coli BL21(DE3)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KOD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6KOD FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6kod FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kod OCA], [https://pdbe.org/6kod PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6kod RCSB], [https://www.ebi.ac.uk/pdbsum/6kod PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6kod ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/RCLA_ECOLI RCLA_ECOLI] Probably involved in reactive chlorine species (RCS) stress resistance.<ref>PMID:24078635</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
In response to microbial invasion, the animal immune system generates hypochlorous acid (HOCl) that kills microorganisms in the oxidative burst. HOCl toxicity is amplified in the phagosome through import of the copper cation (Cu(2+)). In Escherichia coli and Salmonella, the transcriptional regulator RclR senses HOCl stress and induces expression of the RclA, -B, and -C proteins, involved in bacterial defenses against oxidative stress. However, the structures and biochemical roles of the Rcl proteins remain to be elucidated. In this study, we first examined the role of the flavoprotein disulfide reductase (FDR) RclA in survival of Salmonella in macrophage phagosomes, finding that RclA promotes Salmonella survival in macrophage vacuoles containing sublethal HOCl levels. To clarify the molecular mechanism, we determined the crystal structure of RclA from E.coli at 2.9 A resolution. This analysis revealed that the structure of homodimeric RclA is similar to those of typical FDRs, exhibiting two conserved cysteine residues near the flavin ring of the cofactor flavin adenine dinucleotide (FAD). Of note, we observed that Cu(2+) accelerated RclA-mediated oxidation of NADH, leading to a lowering of oxygen levels in vitro Compared with the RclA wildtype enzyme, substitution of the conserved cysteine residues lowered the specificity to Cu(2+) or substantially increased the production of superoxide anion in the absence of Cu(2+) We conclude that RclA-mediated lowering of oxygen levels could contribute to the inhibition of oxidative bursts in phagosomes. Our study sheds light on the molecular basis for how bacteria can survive HOCl stress in macrophages.

Structure and function of the hypochlorous acid-induced flavoprotein RclA from Escherichia coli.,Baek Y, Kim J, Ahn J, Jo I, Hong S, Ryu S, Ha NC J Biol Chem. 2020 Jan 26. pii: RA119.011530. doi: 10.1074/jbc.RA119.011530. PMID:31988242<ref>PMID:31988242</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6kod" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Large Structures]]
[[Category: Baek Y]]
[[Category: Ha N-C]]