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==Crystal structure of Juvenile hormone diol kinase JHDK-L2 from silkworm, Bombyx mori==
<StructureSection load='6kth' size='340' side='right'caption='[[6kth]], [[Resolution|resolution]] 1.22&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6kth]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bombyx_mori Bombyx mori]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KTH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6KTH FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.22&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6kth FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kth OCA], [https://pdbe.org/6kth PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6kth RCSB], [https://www.ebi.ac.uk/pdbsum/6kth PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6kth ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Juvenile hormone diol kinase (JHDK) is an important enzyme involved in the juvenile hormone metabolism pathway, which catalyzes the phosphorylation of juvenile hormone diol to form the polar metabolite JH diol phosphate. Here, we reported the first crystal structure of insect JHDK from Bombyx mori, BmJHDK-L2, determined at a resolution of 1.22 A. The structure of BmJHDK-L2 mainly comprises of eight alpha-helical segments linked with loops, forming four helix-loop-helix motifs. In these four helix-loop-helix motifs with only one calcium ion bound in the first motif. Circular dichroism spectra indicated that BmJHDK-L2 has strong thermal stability, which is independent of the divalent cation. The structure of BmJHDK-L2 further allowed us to define an ATP-binding site using computational simulation and binding assays, providing a structural basis for development of inhibitor of JHDK. Moreover, the expression profile of BmJHDK-L2 indicated a predominant role in juvenile hormone metabolism in the Malpighian tubules of silkworm. Collectively, these findings expand our knowledge regarding the structural and biochemical features of insect JHDK proteins.

Structural characterization and functional analysis of juvenile hormone diol kinase from the silkworm, Bombyx mori.,Xu H, Zhang Y, Zhang L, Wang Z, Guo P, Zhao P Int J Biol Macromol. 2021 Jan 15;167:570-577. doi:, 10.1016/j.ijbiomac.2020.11.138. Epub 2020 Nov 26. PMID:33249150<ref>PMID:33249150</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6kth" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Bombyx mori]]
[[Category: Large Structures]]
[[Category: Guo PC]]
[[Category: Wang Z]]
[[Category: Xu HY]]
[[Category: Zhang L]]
[[Category: Zhang YS]]
[[Category: Zhao P]]