View source for 6ktj


==Crystal structure of scyllo-inositol dehydrogenase R178A mutant, apo-form, from Paracoccus laeviglucosivorans==
<StructureSection load='6ktj' size='340' side='right'caption='[[6ktj]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6ktj]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Paracoccus_laeviglucosivorans Paracoccus laeviglucosivorans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KTJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6KTJ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ktj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ktj OCA], [https://pdbe.org/6ktj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ktj RCSB], [https://www.ebi.ac.uk/pdbsum/6ktj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ktj ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/K7ZP76_9RHOB K7ZP76_9RHOB] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
scyllo-inositol dehydrogenase, isolated from Paracoccus laeviglucosivorans (Pl-sIDH), exhibits a broad substrate specificity: it oxidizes scyllo- and myo-inositols as well as L-glucose, converting L-glucose to L-glucono-1,5-lactone. Based on the crystal structures previously reported, Arg178 residue, located at the entry port of the catalytic site, seemed to be important for accepting substrates. Here, we report the role of Arg178 by using an alanine-substituted mutant for kinetic analysis as well as to determine the crystal structures. The wild-type Pl-sIDH exhibits the activity for scyllo-inositol most preferably followed by myo-inositol and L-glucose. On the contrary, the R178A mutant abolished the activities for both inositols, but remained active for L-glucose to the same extent as its wild-type. Based on the crystal structures of the mutant, the side chain of Asp191 flipped out of the substrate binding site. Therefore, Arg178 is important in positioning Asp191 correctly to exert its catalytic activities.Abbreviations: IDH: inositol dehydrogenase; LB: Luria-Bertani; kcat: catalyst rate constant; Km: Michaelis constant; NAD: nicotinamide dinucleotide; NADH: nicotinamide dinucleotide reduced form; PDB; Protein Data Bank; PDB entry: 6KTJ, 6KTK, 6KTL.

Single amino acid mutation altered substrate specificity for L-glucose and inositol in scyllo-inositol dehydrogenase isolated from Paracoccus laeviglucosivorans.,Suzuki M, Koubara K, Takenoya M, Fukano K, Ito S, Sasaki Y, Nakamura A, Yajima S Biosci Biotechnol Biochem. 2019 Dec 16:1-9. doi: 10.1080/09168451.2019.1702870. PMID:31842701<ref>PMID:31842701</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6ktj" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Paracoccus laeviglucosivorans]]
[[Category: Fukano K]]
[[Category: Ito S]]
[[Category: Koubara K]]
[[Category: Nakamura A]]
[[Category: Sasaki Y]]
[[Category: Suzuki M]]
[[Category: Takenoya M]]
[[Category: Yajima S]]