View source for 6kvc


==MoeE5 in complex with UDP-glucose and NAD==
<StructureSection load='6kvc' size='340' side='right'caption='[[6kvc]], [[Resolution|resolution]] 1.66&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6kvc]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_viridosporus_ATCC_14672 Streptomyces viridosporus ATCC 14672]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KVC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6KVC FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.66&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAD:NICOTINAMIDE-ADENINE-DINUCLEOTIDE'>NAD</scene>, <scene name='pdbligand=UPG:URIDINE-5-DIPHOSPHATE-GLUCOSE'>UPG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6kvc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kvc OCA], [https://pdbe.org/6kvc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6kvc RCSB], [https://www.ebi.ac.uk/pdbsum/6kvc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6kvc ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A003_STRVD A0A003_STRVD] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The epimerase MoeE5 from Streptomyces viridosporus converts UDP-glucuronic acid (UDP-GlcA) to UDP-galacturonic acid (UDP-GalA) to provide the first sugar in synthesizing moenomycin, a potent inhibitor against bacterial peptidoglycan glycosyltransferases. The enzyme belongs to the UDP-hexose 4-epimerase family, and uses NAD(+) as its cofactor. Here we present the complex crystal structures of MoeE5/NAD(+)/UDP-GlcA and MoeE5/NAD(+)/UDP-glucose, determined at 1.48A and 1.66A resolution. The cofactor NAD(+) is bound to the N-terminal Rossmann-fold domain and the substrate is bound to the smaller C-terminal domain. In both crystals the C4 atom of the sugar moiety of the substrate is in close proximity to the C4 atom of the nicotinamide of NAD(+), and the O4 atom of the sugar is also hydrogen bonded to the side chain of Tyr154, suggesting a productive binding mode. As the first complex structure of this protein family with a bound UDP-GlcA in the active site, it shows an extensive hydrogen-bond network between the enzyme and the substrate. We further built a model with the product UDP-GalA, and found that the unique Arg192 of MoeE5 might play an important role in the catalytic pathway. Consequently, MoeE5 is likely a specific epimerase for UDP-GlcA to UDP-GalA conversion, rather than a promiscuous enzyme as some other family members.

Structure of an antibiotic-synthesizing UDP-glucuronate 4-epimerase MoeE5 in complex with substrate.,Sun H, Ko TP, Liu W, Liu W, Zheng Y, Chen CC, Guo RT Biochem Biophys Res Commun. 2019 Oct 22. pii: S0006-291X(19)31924-2. doi:, 10.1016/j.bbrc.2019.10.035. PMID:31653344<ref>PMID:31653344</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6kvc" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Streptomyces viridosporus ATCC 14672]]
[[Category: Chen C-C]]
[[Category: Guo R-T]]
[[Category: Ko T-P]]
[[Category: Liu W]]
[[Category: Sun H]]