View source for 6kvk


==Crystal structure of UDP-Sm-SrUGT76G1==
<StructureSection load='6kvk' size='340' side='right'caption='[[6kvk]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6kvk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Stevia_rebaudiana Stevia rebaudiana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KVK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6KVK FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.397&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DX0:Steviolmonoside'>DX0</scene>, <scene name='pdbligand=UDP:URIDINE-5-DIPHOSPHATE'>UDP</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6kvk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kvk OCA], [https://pdbe.org/6kvk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6kvk RCSB], [https://www.ebi.ac.uk/pdbsum/6kvk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6kvk ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/U76G1_STERE U76G1_STERE] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Diterpene glycosyltransferase UGT76G1 from Stevia rebaudiana (SrUGT76G1) is key to the generation of economically important steviol glycosides (SGs), a group of natural sweeteners with high-intensity sweetness. SrUGT76G1 accommodates a wide range of steviol-derived substrates and many other small molecules. We report here the crystal structures of SrUGT76G1 in complex with multiple ligands to answer how this enzyme recognizes diterpenoid aglycones and catalyzes the 1,3-sugar chain branching. A spacious pocket for sugar-acceptor binding was observed from the determined SrUGT76G1 structures, which can explain its broad substrate spectrum. Residues Gly87 and Leu204 lining the pocket play key roles in switching between diterpenoid and flavonoid glucosylation. An engineered mutant of SrUGT76G1, T284S, could catalyze a selectively increased production of next-generation sweetener rebaudioside M, with diminished side product of rebaudioside I. Taken together, these resutls provide significant insights into molecular basis of the substrate specificity of scarcely documented diterpenoid glycosyltransferases and would facilitate the structure-guided glycoengineering to produce diversified diterpenoids with new activities.

Structural Insights into the Catalytic Mechanism of a Plant Diterpene Glycosyltransferase SrUGT76G1.,Liu Z, Li J, Sun Y, Zhang P, Wang Y Plant Commun. 2019 Sep 28;1(1):100004. doi: 10.1016/j.xplc.2019.100004., eCollection 2020 Jan 13. PMID:33404544<ref>PMID:33404544</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6kvk" style="background-color:#fffaf0;"></div>

==See Also==
*[[Glycosyltransferase 3D structures|Glycosyltransferase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Stevia rebaudiana]]
[[Category: Li JX]]
[[Category: Liu ZF]]
[[Category: Wang Y]]
[[Category: Zhang P]]