View source for 6kyb


==Crystal structure of Atg18 from Saccharomyces cerevisiae==
<StructureSection load='6kyb' size='340' side='right'caption='[[6kyb]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6kyb]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6KYB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6KYB FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6kyb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6kyb OCA], [https://pdbe.org/6kyb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6kyb RCSB], [https://www.ebi.ac.uk/pdbsum/6kyb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6kyb ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/ATG18_YEAST ATG18_YEAST] The PI(3,5)P2 regulatory complex regulates both the synthesis and turnover of phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). May negatively regulate FAB1 activity by sequestering or masking VAC7 from FAB1. Necessary for proper vacuole morphology. Plays an important role in osmotically-induced vacuole fragmentation. Required for cytoplasm to vacuole transport (Cvt) vesicle formation, pexophagy and starvation-induced autophagy. Involved in correct ATG9 trafficking to the pre-autophagosomal structure. Might also be involved in premeiotic DNA replication. With ATG2, protects ATG8 from ATG4-mediated cleavage.<ref>PMID:11470404</ref> <ref>PMID:11536337</ref> <ref>PMID:11707261</ref> <ref>PMID:11739783</ref> <ref>PMID:14723849</ref> <ref>PMID:15155809</ref> <ref>PMID:15194695</ref> <ref>PMID:16876790</ref> <ref>PMID:17699591</ref> <ref>PMID:18586673</ref> <ref>PMID:18769150</ref> <ref>PMID:19037259</ref> <ref>PMID:20154084</ref> <ref>PMID:22108003</ref> <ref>PMID:22728243</ref> <ref>PMID:22787281</ref> <ref>PMID:24905091</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Macroautophagy (hereafter referred to as autophagy) is a highly conserved catabolic eukaryotic pathway that is critical for stress responses and homeostasis. Atg18, one of the core proteins involved in autophagy, belongs to the PROPPIN family and is composed of seven WD40 repeats. Together with Atg2, Atg18 participates in the elongation of phagophores and the recycling of Atg9 in yeast. Despite extensive studies on the PROPPIN family, the structure of Atg18 from Saccharomyces cerevisiae has not been determined. Here, we report the structure of ScAtg18 at a resolution of 2.8 A. Based on bioinformatics and structural analysis, we found that the 7AB loop of ScAtg18 is extended in Atg18, in comparison to other members of the PROPPIN family. Genetic analysis revealed that the 7AB loop of ScAtg18 is required for autophagy. Biochemical and biophysical experiments indicated that the 7AB loop of ScAtg18 is critical for interaction with ScAtg2 and the recruitment of ScAtg2 to the autophagy-initiating site. Collectively, our results show that the 7AB loop of ScAtg18 is a new binding site for Atg2 and is of functional importance to autophagy.

The crystal structure of Atg18 reveals a new binding site for Atg2 in Saccharomyces cerevisiae.,Lei Y, Tang D, Liao G, Xu L, Liu S, Chen Q, Li C, Duan J, Wang K, Wang J, Sun B, Li Z, Dai L, Cheng W, Qi S, Lu K Cell Mol Life Sci. 2020 Aug 18. pii: 10.1007/s00018-020-03621-9. doi:, 10.1007/s00018-020-03621-9. PMID:32809042<ref>PMID:32809042</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6kyb" style="background-color:#fffaf0;"></div>

==See Also==
*[[Autophagy-related protein 3D structures|Autophagy-related protein 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae S288C]]
[[Category: Chen Q]]
[[Category: Lei Y]]
[[Category: Liao G]]
[[Category: Lu K]]
[[Category: Qi S]]
[[Category: Tang D]]
[[Category: Xu L]]