View source for 6l70


==Complex structure of PEDV 3CLpro with GC376==
<StructureSection load='6l70' size='340' side='right'caption='[[6l70]], [[Resolution|resolution]] 1.56&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6l70]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Porcine_epidemic_diarrhea_virus Porcine epidemic diarrhea virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6L70 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6L70 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.56&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=K36:(1S,2S)-2-({N-[(BENZYLOXY)CARBONYL]-L-LEUCYL}AMINO)-1-HYDROXY-3-[(3S)-2-OXOPYRROLIDIN-3-YL]PROPANE-1-SULFONIC+ACID'>K36</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6l70 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6l70 OCA], [https://pdbe.org/6l70 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6l70 RCSB], [https://www.ebi.ac.uk/pdbsum/6l70 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6l70 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/K4L9I6_9ALPC K4L9I6_9ALPC] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Porcine epidemic diarrhea virus (PEDV), being highly virulent and contagious in piglets, has caused significant damage to the pork industries of many countries worldwide. There are no commercial drugs targeting coronaviruses (CoVs), and few studies on anti-PEDV inhibitors. The coronavirus 3C-like protease (3CL(pro)) has a conserved structure and catalytic mechanism and plays a key role during viral polyprotein processing, thus serving as an appealing antiviral drug target. Here, we report the anti-PEDV effect of the broad-spectrum inhibitor GC376 (targeting 3Cpro or 3CL(pro) of viruses in the picornavirus-like supercluster). GC376 was highly effective against the PEDV 3CL(pro) and exerted similar inhibitory effects on two PEDV strains. Furthermore, the structure of the PEDV 3CL(pro) in complex with GC376 was determined at 1.65 A. We elucidated structural details and analyzed the differences between GC376 binding with the PEDV 3CL(pro) and GC376 binding with the transmissible gastroenteritis virus (TGEV) 3CL(pro). Finally, we explored the substrate specificity of PEDV 3CL(pro) at the P2 site and analyzed the effects of Leu group modification in GC376 on inhibiting PEDV infection. This study helps us to understand better the PEDV 3CL(pro) substrate specificity, providing information on the optimization of GC376 for development as an antiviral therapeutic against coronaviruses.

Structural Basis for Inhibiting Porcine Epidemic Diarrhea Virus Replication with the 3C-Like Protease Inhibitor GC376.,Ye G, Wang X, Tong X, Shi Y, Fu ZF, Peng G Viruses. 2020 Feb 21;12(2). pii: v12020240. doi: 10.3390/v12020240. PMID:32098094<ref>PMID:32098094</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6l70" style="background-color:#fffaf0;"></div>

==See Also==
*[[Virus protease 3D structures|Virus protease 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Porcine epidemic diarrhea virus]]
[[Category: Peng GQ]]
[[Category: Ye G]]