View source for 6l86


==The structure of SfaA==
<StructureSection load='6l86' size='340' side='right'caption='[[6l86]], [[Resolution|resolution]] 2.23&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6l86]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_thioluteus Streptomyces thioluteus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6L86 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6L86 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.23&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=LMR:(2S)-2-HYDROXYBUTANEDIOIC+ACID'>LMR</scene>, <scene name='pdbligand=MLT:D-MALATE'>MLT</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6l86 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6l86 OCA], [https://pdbe.org/6l86 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6l86 RCSB], [https://www.ebi.ac.uk/pdbsum/6l86 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6l86 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A2H4T920_STRTU A0A2H4T920_STRTU] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
N-alkylisonitrile, a precursor to isonitrile-containing lipopeptides, is biosynthesized via decarboxylation-assisted -N identical withC group (isonitrile) formation by using N-alkylglycine as the substrate. This reaction is catalyzed by iron(II) and 2-oxoglutarate (Fe/2OG) dependent enzymes. Distinct from typical oxygenation or halogenation reactions catalyzed by this class of enzymes, installation of the isonitrile group represents a novel reaction type for Fe/2OG enzymes that involves a four-electron oxidative process. Here we report the plausible mechanism of three Fe/2OG enzymes, Sav607, ScoE and SfaA, catalyzed isonitrile formation. The X-ray structures of iron loaded ScoE in complex with its substrate and the intermediate, along with biochemical and biophysical data reveal that -N identical withC bond formation involves two cycles of Fe/2OG enzyme catalysis. The reaction starts with an Fe(IV)-oxo catalyzed hydroxylation. It is likely followed by decarboxylation-assisted desaturation to complete isonitrile installation.

Pathway from N-alkylglycine to alkylisonitrile catalyzed by iron(II) and 2-oxoglutarate dependent oxygenases.,Chang WC, Chen TY, Chen J, Tang Y, Zhou J, Guo Y Angew Chem Int Ed Engl. 2020 Feb 19. doi: 10.1002/anie.201914896. PMID:32074393<ref>PMID:32074393</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6l86" style="background-color:#fffaf0;"></div>

==See Also==
*[[Dioxygenase 3D structures|Dioxygenase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Streptomyces thioluteus]]
[[Category: Chang W]]
[[Category: Chen J]]
[[Category: Chen TY]]
[[Category: Zhou J]]