View source for 6lbt


==Crystal structure of yeast Cdc13 and Stn1==
<StructureSection load='6lbt' size='340' side='right'caption='[[6lbt]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6lbt]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Kluyveromyces_lactis Kluyveromyces lactis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LBT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6LBT FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6lbt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6lbt OCA], [https://pdbe.org/6lbt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6lbt RCSB], [https://www.ebi.ac.uk/pdbsum/6lbt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6lbt ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q6CJ70_KLULA Q6CJ70_KLULA] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Budding yeast Cdc13-Stn1-Ten1 (CST) complex plays an essential role in telomere protection and maintenance. Despite extensive studies, only structural information of individual domains of CST is available; the architecture of CST still remains unclear. Here, we report crystal structures of Kluyveromyces lactis Cdc13-telomeric-DNA, Cdc13-Stn1 and Stn1-Ten1 complexes and propose an integrated model depicting how CST assembles and plays its roles at telomeres. Surprisingly, two oligonucleotide/oligosaccharide-binding (OB) folds of Cdc13 (OB2 and OB4), previously believed to mediate Cdc13 homodimerization, actually form a stable intramolecular interaction. This OB2-OB4 module of Cdc13 is required for the Cdc13-Stn1 interaction that assembles CST into an architecture with a central ring-like core and multiple peripheral modules in a 2:2:2 stoichiometry. Functional analyses indicate that this unique CST architecture is essential for both telomere capping and homeostasis regulation. Overall, our results provide fundamentally valuable structural information regarding the CST complex and its roles in telomere biology.

Structural insights into telomere protection and homeostasis regulation by yeast CST complex.,Ge Y, Wu Z, Chen H, Zhong Q, Shi S, Li G, Wu J, Lei M Nat Struct Mol Biol. 2020 Jul 13. pii: 10.1038/s41594-020-0459-8. doi:, 10.1038/s41594-020-0459-8. PMID:32661422<ref>PMID:32661422</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6lbt" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Kluyveromyces lactis]]
[[Category: Large Structures]]
[[Category: Ge Y]]
[[Category: Lei M]]
[[Category: Wu J]]
[[Category: Wu Z]]