View source for 6lct


==Crystal structure of catalytic inactive chloroplast resolvase NtMOC1 in complex with Holliday junction==
<StructureSection load='6lct' size='340' side='right'caption='[[6lct]], [[Resolution|resolution]] 2.55&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6lct]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Nicotiana_tabacum Nicotiana tabacum] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LCT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6LCT FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.55&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6lct FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6lct OCA], [https://pdbe.org/6lct PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6lct RCSB], [https://www.ebi.ac.uk/pdbsum/6lct PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6lct ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A1S4CVP6_TOBAC A0A1S4CVP6_TOBAC] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Holliday junctions (HJs) are key DNA intermediates in genetic recombination and are eliminated by nuclease, termed resolvase, to ensure genome stability. HJ resolvases have been identified across all kingdoms of life, members of which exhibit sequence-dependent HJ resolution. However, the molecular basis of sequence selectivity remains largely unknown. Here, we present the chloroplast resolvase MOC1, which cleaves HJ in a cytosine-dependent manner. We determine the crystal structure of MOC1 with and without HJs. MOC1 exhibits an RNase H fold, belonging to the retroviral integrase family. MOC1 functions as a dimer, and the HJ is embedded into the basic cleft of the dimeric enzyme. We characterize a base recognition loop (BR loop) that protrudes into and opens the junction. Residues from the BR loop intercalate into the bases, disrupt the C-G base pairing at the crossover and recognize the cytosine, providing the molecular basis for sequence-dependent HJ resolution by a resolvase.

Structural insights into sequence-dependent Holliday junction resolution by the chloroplast resolvase MOC1.,Yan J, Hong S, Guan Z, He W, Zhang D, Yin P Nat Commun. 2020 Mar 17;11(1):1417. doi: 10.1038/s41467-020-15242-8. PMID:32184398<ref>PMID:32184398</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6lct" style="background-color:#fffaf0;"></div>

==See Also==
*[[Resolvase 3D structures|Resolvase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Nicotiana tabacum]]
[[Category: Synthetic construct]]
[[Category: Guan ZY]]
[[Category: Hong SX]]
[[Category: Yan JJ]]
[[Category: Yin P]]