View source for 6lek


==Tertiary structure of Barnacle cement protein MrCP20==
<StructureSection load='6lek' size='340' side='right'caption='[[6lek]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6lek]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Megabalanus_rosa Megabalanus rosa]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LEK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6LEK FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 10 models</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6lek FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6lek OCA], [https://pdbe.org/6lek PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6lek RCSB], [https://www.ebi.ac.uk/pdbsum/6lek PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6lek ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/CEM20_MEGRO CEM20_MEGRO] Component of the insoluble proteinaceous cement which allows underwater adhesion to rocks and other surfaces (PubMed:11368778, PubMed:18021251). Shows specific adsorption on calcite substrates (PubMed:18021251).<ref>PMID:11368778</ref> <ref>PMID:18021251</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Barnacles employ a protein-based cement to firmly attach to immersed substrates. The cement proteins (CPs) have previously been identified and sequenced. However, the molecular mechanisms of adhesion are not well understood, in particular, because the three-dimensional molecular structure of CPs remained unknown to date. Here, we conducted multi-dimensional nuclear magnetic resonance (NMR) studies and molecular dynamics (MD) simulations of recombinant Megabalanus rosa Cement Protein 20 (rMrCP20). Our NMR results show that rMrCP20 contains three main folded domain regions intervened by two dynamic loops, resulting in multiple protein conformations that exist in equilibrium. We found that 12 out of 32 Cys in the sequence engage in disulfide bonds that stabilize the beta-sheet domains owing to their placement at the extremities of beta-strands. Another feature unveiled by NMR is the location of basic residues in turn regions that are exposed to the solvent, playing an important role for intermolecular contact with negatively charged surfaces. MD simulations highlight a highly stable and conserved beta-motif (beta7-beta8), which may function as nuclei for amyloid-like nanofibrils previously observed in the cured adhesive cement. To the best of our knowledge, this is the first report describing the tertiary structure of an extracellular biological adhesive protein at the molecular level. This article is part of the theme issue 'Transdisciplinary approaches to the study of adhesion and adhesives in biological systems'.

Three-dimensional structure of Megabalanus rosa Cement Protein 20 revealed by multi-dimensional NMR and molecular dynamics simulations.,Mohanram H, Kumar A, Verma CS, Pervushin K, Miserez A Philos Trans R Soc Lond B Biol Sci. 2019 Oct 28;374(1784):20190198. doi: , 10.1098/rstb.2019.0198. Epub 2019 Sep 9. PMID:31495314<ref>PMID:31495314</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6lek" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Megabalanus rosa]]
[[Category: Mohanram H]]