View source for 6lhl


==The cryo-EM structure of coxsackievirus A16 A-particle in complex with Fab 18A7==
<SX load='6lhl' size='340' side='right' viewer='molstar' caption='[[6lhl]], [[Resolution|resolution]] 3.07&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6lhl]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Coxsackievirus_A16 Coxsackievirus A16]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LHL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6LHL FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.07&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6lhl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6lhl OCA], [https://pdbe.org/6lhl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6lhl RCSB], [https://www.ebi.ac.uk/pdbsum/6lhl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6lhl ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A2S1BJ89_9ENTO A0A2S1BJ89_9ENTO] 
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== Publication Abstract from PubMed ==
Hand, foot, and mouth disease is a common childhood illness primarily caused by coxsackievirus A16 (CVA16), for which there are no current vaccines or treatments. We identify three CVA16-specific neutralizing monoclonal antibodies (nAbs) with therapeutic potential: 18A7, 14B10, and NA9D7. We present atomic structures of these nAbs bound to all three viral particle forms-the mature virion, A-particle, and empty particle-and show that each Fab can simultaneously occupy the mature virion. Additionally, 14B10 or NA9D7 provide 100% protection against lethal CVA16 infection in a neonatal mouse model. 18A7 binds to a non-conserved epitope present in all three particles, whereas 14B10 and NA9D7 recognize broad protective epitopes but only bind the mature virion. NA9D7 targets an immunodominant site, which may overlap the receptor-binding site. These findings indicate that CVA16 vaccines should be based on mature virions and that these antibodies could be used to discriminate optimal virion-based immunogens.

Identification of Antibodies with Non-overlapping Neutralization Sites that Target Coxsackievirus A16.,He M, Xu L, Zheng Q, Zhu R, Yin Z, Zha Z, Lin Y, Yang L, Huang Y, Ye X, Li S, Hou W, Wu Y, Han J, Liu D, Li Z, Chen Z, Yu H, Que Y, Wang Y, Yan X, Zhang J, Gu Y, Zhou ZH, Cheng T, Li S, Xia N Cell Host Microbe. 2020 Feb 12;27(2):249-261.e5. doi: 10.1016/j.chom.2020.01.003., Epub 2020 Feb 5. PMID:32027857<ref>PMID:32027857</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 6lhl" style="background-color:#fffaf0;"></div>

==See Also==
*[[Virus coat proteins 3D structures|Virus coat proteins 3D structures]]
== References ==
<references/>
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[[Category: Coxsackievirus A16]]
[[Category: Large Structures]]
[[Category: Cheng T]]
[[Category: He MZ]]
[[Category: Li SW]]
[[Category: Xu LF]]
[[Category: Yin ZC]]
[[Category: Zheng QB]]
[[Category: Zhu R]]