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==Crystal Structure of the peptidylprolyl isomerase domain of Arabidopsis thaliana CYP71.==
<StructureSection load='6lkb' size='340' side='right'caption='[[6lkb]], [[Resolution|resolution]] 1.65&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6lkb]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LKB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6LKB FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.651&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6lkb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6lkb OCA], [https://pdbe.org/6lkb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6lkb RCSB], [https://www.ebi.ac.uk/pdbsum/6lkb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6lkb ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/CPY71_ARATH CPY71_ARATH] PPIases accelerate the folding of proteins (Probable). It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides (PubMed:33098102). Histone proline isomerase that increases the rate of cis-trans isomerization of the synthetic histone H3 peptides H3P30 (RKSAP30F-p-nitroanilide) and H3P30K27me3 (RKme3-SAP30F-p-nitroanilide) in the histone H3 N-terminal tail, in vitro (PubMed:33098102). Histone remodeling factor involved in chromatin-based gene silencing (PubMed:17704213). Reinforces H3K27 methylation (PubMed:17704213). Involved in fundamental processes of chromatin assembly and histone modification by mediating the targeting of FAS1 and LHP1 on the chromatin (PubMed:21596687). Required for the formation and development of leaves, for normal phyllotaxy and for the formation, maintenance and activity of root and shoot apical meristems (PubMed:17704213).<ref>PMID:17704213</ref> <ref>PMID:21596687</ref> <ref>PMID:33098102</ref> <ref>PMID:17704213</ref> <ref>PMID:21596687</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Arabidopsis thaliana CYP71 (AtCYP71) is a chromatin-remodeling protein that promotes shoot apical meristem (SAM) differentiation. The N terminus of AtCYP71 contains a noncanonical WD domain, and the C terminus contains an enzymatic peptidyl-prolyl isomerase (PPIase) cyclophilin (CYP) domain. To date, there has been no characterization of CYP71, and its mode of action remains unknown. Here, we report the crystal structure of the CYP domain of AtCYP71 at 1.9 A resolution. The structure shows key differences when compared to the canonical CYP fold of human CypA. To the best our knowledge, this is the first A. thaliana CYP structure with a conserved active site loop. Using nuclear magnetic resonance spectroscopy, we demonstrate that the CYP domain is active toward histone H3. Our findings suggest that the PPIase activity of the CYP domain is important for the function of AtCYP71 in chromatin remodeling during organogenesis.

Structural and functional analyses of the PPIase domain of Arabidopsis thaliana CYP71 reveal its catalytic activity toward histone H3.,Lakhanpal S, Fan JS, Luan S, Swaminathan K FEBS Lett. 2020 Oct 23. doi: 10.1002/1873-3468.13965. PMID:33098102<ref>PMID:33098102</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6lkb" style="background-color:#fffaf0;"></div>

==See Also==
*[[Peptidyl-prolyl cis-trans isomerase 3D structures|Peptidyl-prolyl cis-trans isomerase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Arabidopsis thaliana]]
[[Category: Large Structures]]
[[Category: Jobichen C]]
[[Category: Lakhanpal S]]
[[Category: Swaminathan K]]