View source for 6lpw


==Structure of Spermidine disinapoyl transferases(SDT) from Arabidopsis thaliana==
<StructureSection load='6lpw' size='340' side='right'caption='[[6lpw]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6lpw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LPW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6LPW FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.401&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SPD:SPERMIDINE'>SPD</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6lpw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6lpw OCA], [https://pdbe.org/6lpw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6lpw RCSB], [https://www.ebi.ac.uk/pdbsum/6lpw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6lpw ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/SDT_ARATH SDT_ARATH] Spermidine sinapoyl-CoA acyltransferase that mediates the accumulation of disinapoyl spermidine conjugates in seeds (PubMed:19168716). Catalyzes the two conjugating steps required for the biosynthesis of N1,N8-disipanoyl-spermidine (PubMed:19168716, PubMed:33519864). Can also use putrescine as an acyl acceptor to convert it into monosinapoyl-putrescine (PubMed:19168716).<ref>PMID:19168716</ref> <ref>PMID:33519864</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Phenolamides represent one of the largest classes of plant-specialized secondary metabolites and function in diverse physiological processes, including defense responses and development. The biosynthesis of phenolamides requires the BAHD-family acyltransferases, which transfer acyl-groups from different acyl-donors specifically to amines, the acyl-group acceptors. However, the mechanisms of substrate specificity and multisite-acylation of the BAHD-family acyltransferases remain poorly understood. In this study, we provide a structural and biochemical analysis of AtSHT and AtSDT, two representative BAHD-family members that catalyze the multisite acylation of spermidine but show different product profiles. By determining the structures of AtSHT and AtSDT and using structure-based mutagenesis, we identified the residues important for substrate recognition in AtSHT and AtSDT and hypothesized that the acyl acceptor spermidine might adopt a free-rotating conformation in AtSHT, which can undergo mono-, di-, or tri-acylation; while the spermidine molecule in AtSDT might adopt a linear conformation, which only allows mono- or di-acylation to take place. In addition, through sequence similarity network (SSN) and structural modeling analysis, we successfully predicted and verified the functions of two uncharacterized Arabidopsis BAHD acyltransferases, OAO95042.1 and NP_190301.2, which use putrescine as the main acyl-acceptor. Our work provides not only an excellent starting point for understanding multisite acylation in BAHD-family enzymes, but also a feasible methodology for predicting possible acyl acceptor specificity of uncharacterized BAHD-family acyltransferases.

Structural and Biochemical Insights Into Two BAHD Acyltransferases (AtSHT and AtSDT) Involved in Phenolamide Biosynthesis.,Wang C, Li J, Ma M, Lin Z, Hu W, Lin W, Zhang P Front Plant Sci. 2021 Jan 13;11:610118. doi: 10.3389/fpls.2020.610118. , eCollection 2020. PMID:33519864<ref>PMID:33519864</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6lpw" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Arabidopsis thaliana]]
[[Category: Large Structures]]
[[Category: Wang CY]]
[[Category: Zhang P]]