View source for 6lqk


==Crystal structure of honeybee RyR NTD==
<StructureSection load='6lqk' size='340' side='right'caption='[[6lqk]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6LQK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6LQK FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.499&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6lqk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6lqk OCA], [https://pdbe.org/6lqk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6lqk RCSB], [https://www.ebi.ac.uk/pdbsum/6lqk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6lqk ProSAT]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
Ryanodine receptors (RyRs) are the molecular target of diamides, a new chemical class of insecticides. Diamide insecticides are used to control lepidopteran pests and were considered relatively safe for mammals and non-targeted beneficial insects, including honey bees. However, recent studies showed that exposure to diamides could cause long-lasting locomotor deficits of bees. Here we report the crystal structure of RyR N-terminal domain A (NTD-A) from the honeybee, Apis mellifera, at 2.5 A resolution. It shows a similar overall fold as the RyR NTD-A from mammals and the diamondback moth (DBM), Plutella xylostella, and still several loops located at the inter-domain interfaces show insect-specific or bee-specific structural features. A potential insecticide-binding pocket formed by loop9 and loop13 is conserved in lepidopteran but different in both mammals and bees, making it a good candidate targeting site for the development of pest-selective insecticides. Furthermore, a conserved intra-domain disulfide bond was observed in both DBM and bee RyR NTD-A crystal structures, which explains their higher thermal stability compared to mammalian RyR NTD-A. This work provides a basis for the development of novel insecticides with better selectivity between pests and bees by targeting a distinct site on pest RyRs, which would be a promising strategy to overcome the current toxicity problem.

Crystal structure of the N-terminal domain of ryanodine receptor from the honeybee, Apis mellifera.,Zhou Y, Wang W, Salauddin NM, Lin L, You M, You S, Yuchi Z Insect Biochem Mol Biol. 2020 Oct;125:103454. doi: 10.1016/j.ibmb.2020.103454., Epub 2020 Aug 8. PMID:32781205<ref>PMID:32781205</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 6lqk" style="background-color:#fffaf0;"></div>

==See Also==
*[[Ryanodine receptor 3D structures|Ryanodine receptor 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Lin L]]
[[Category: Yuchi Z]]
[[Category: Zhou Y]]