View source for 6m2i


==Structure of the 2-Aminoisobutyric acid Monooxygenase Hydroxylase==
<StructureSection load='6m2i' size='340' side='right'caption='[[6m2i]], [[Resolution|resolution]] 2.45&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6m2i]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Rhodococcus_wratislaviensis Rhodococcus wratislaviensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6M2I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6M2I FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.45&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6m2i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6m2i OCA], [https://pdbe.org/6m2i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6m2i RCSB], [https://www.ebi.ac.uk/pdbsum/6m2i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6m2i ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A402C2V4_RHOWR A0A402C2V4_RHOWR] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The high-valent iron-oxo species formed in the non-heme diiron enzymes have high oxidative reactivity and catalyze difficult chemical reactions. Although the hydroxylation of inert methyl groups is an industrially promising reaction, utilizing non-heme diiron enzymes as such a biocatalyst has been difficult. Here we show a three-component monooxygenase system for the selective terminal hydroxylation of alpha-aminoisobutyric acid (Aib) into alpha-methyl-D-serine. It consists of the hydroxylase component, AibH1H2, and the electron transfer component. Aib hydroxylation is the initial step of Aib catabolism in Rhodococcus wratislaviensis C31-06, which has been fully elucidated through a proteome analysis. The crystal structure analysis revealed that AibH1H2 forms a heterotetramer of two amidohydrolase superfamily proteins, of which AibHm2 is a non-heme diiron protein and functions as a catalytic subunit. The Aib monooxygenase was demonstrated to be a promising biocatalyst that is suitable for bioprocesses in which the inert C-H bond in methyl groups need to be activated.

A three-component monooxygenase from Rhodococcus wratislaviensis may expand industrial applications of bacterial enzymes.,Hibi M, Fukuda D, Kenchu C, Nojiri M, Hara R, Takeuchi M, Aburaya S, Aoki W, Mizutani K, Yasohara Y, Ueda M, Mikami B, Takahashi S, Ogawa J Commun Biol. 2021 Jan 4;4(1):16. doi: 10.1038/s42003-020-01555-3. PMID:33398074<ref>PMID:33398074</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6m2i" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Large Structures]]
[[Category: Rhodococcus wratislaviensis]]
[[Category: Hibi M]]
[[Category: Mikami B]]
[[Category: Ogawa J]]