View source for 6mb2


==Cryo-EM structure of the PYD filament of AIM2==
<SX load='6mb2' size='340' side='right' viewer='molstar' caption='[[6mb2]], [[Resolution|resolution]] 5.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6mb2]] is a 30 chain structure with sequence from [https://en.wikipedia.org/wiki/Aequorea_victoria Aequorea victoria] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MB2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6MB2 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 5&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CRO:{2-[(1R,2R)-1-AMINO-2-HYDROXYPROPYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>CRO</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6mb2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mb2 OCA], [https://pdbe.org/6mb2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6mb2 RCSB], [https://www.ebi.ac.uk/pdbsum/6mb2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6mb2 ProSAT]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
Absent in melanoma 2 (AIM2) is an essential cytosolic double-stranded DNA receptor that assembles with the adaptor, apoptosis-associated speck-like protein containing a caspase recruitment domain (ASC), and caspase-1 to form the AIM2 inflammasome, which leads to proteolytic maturation of cytokines and pyroptotic cell death. AIM2 contains an N-terminal Pyrin domain (PYD) that interacts with ASC through PYD/PYD interactions and nucleates ASC(PYD) filament formation. To elucidate the molecular basis of AIM2-induced ASC(PYD) polymerization, we generated AIM2(PYD) filaments fused to green fluorescent protein (GFP) and determined its cryo-electron microscopic (cryo-EM) structure. The map showed distinct definition of helices, allowing fitting of the crystal structure. Surprisingly, the GFP-AIM2(PYD) filament is a 1-start helix with helical parameters distinct from those of the 3-start ASC(PYD) filament. However, despite the apparent symmetry difference, helical net and detailed interface analyses reveal minimal changes in subunit packing. GFP-AIM2(PYD) nucleated ASC(PYD) filament formation in comparable efficiency as untagged AIM2(PYD), suggesting assembly plasticity in both AIM2(PYD) and ASC(PYD). The DNA-binding domain of AIM2 is able to form AIM2/DNA filaments, within which the AIM2(PYD) is brought into proximity to template ASC(PYD) filament assembly. Because ASC is able to interact with many PYD-containing receptors for the formation of inflammasomes, the observed structural plasticity may be critically important for this versatility in the PYD/PYD interactions.

Plasticity in PYD assembly revealed by cryo-EM structure of the PYD filament of AIM2.,Lu A, Li Y, Yin Q, Ruan J, Yu X, Egelman E, Wu H Cell Discov. 2015;1. doi: 10.1038/celldisc.2015.13. Epub 2015 Jun 23. PMID:26583071<ref>PMID:26583071</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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==See Also==
*[[Green Fluorescent Protein 3D structures|Green Fluorescent Protein 3D structures]]
== References ==
<references/>
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[[Category: Aequorea victoria]]
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Li Y]]
[[Category: Lu A]]
[[Category: Wu H]]