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==Solution structure of the large extracellular loop of FtsX in Streptococcus pneumoniae==
<StructureSection load='6mk7' size='340' side='right'caption='[[6mk7]]' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6mk7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_pneumoniae_D39 Streptococcus pneumoniae D39]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MK7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6MK7 FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6mk7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mk7 OCA], [https://pdbe.org/6mk7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6mk7 RCSB], [https://www.ebi.ac.uk/pdbsum/6mk7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6mk7 ProSAT]</span></td></tr>
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== Function ==
[https://www.uniprot.org/uniprot/FTSX_STRP2 FTSX_STRP2] Part of the ABC transporter FtsEX involved in asymmetric cellular division facilitating the initiation of sporulation.[UniProtKB:O34876]
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== Publication Abstract from PubMed ==
Streptococcus pneumoniae is a leading killer of infants and immunocompromised adults and has become increasingly resistant to major antibiotics. Therefore, the development of new antibiotic strategies is desperately needed. Targeting bacterial cell division is one such strategy, specifically by targeting proteins that are essential for the synthesis and breakdown of peptidoglycan. One complex important to this process is FtsEX. FtsEX comprises a cell division-regulating integral membrane protein (FtsX) and a cytoplasmic ATPase (FtsE) that resembles an ATP-binding cassette (ABC) transporter. Here, we present nuclear magnetic resonance (NMR) solution structural and crystallographic models of the large extracellular domain of FtsX, denoted extracellular loop 1 (ECL1). The structure of ECL1 reveals an upper extended beta-hairpin and a lower alpha-helical lobe, each extending from a mixed alpha-beta core. The helical lobe mediates a physical interaction with the peptidoglycan hydrolase PcsB via the coiled-coil domain of PcsB (PscBCC). Characterization of S. pneumoniae strain D39-derived strains harboring mutations in the alpha-helical lobe shows that this subdomain is essential for cell viability and required for proper cell division of S. pneumoniae IMPORTANCE FtsX is a ubiquitous bacterial integral membrane protein involved in cell division that regulates the activity of peptidoglycan (PG) hydrolases. FtsX is representative of a large group of ABC3 superfamily proteins that function as "mechanotransmitters," proteins that relay signals from the inside to the outside of the cell. Here, we present a structural characterization of the large extracellular loop, ECL1, of FtsX from the opportunistic human pathogen S. pneumoniae We show the molecular nature of the direct interaction between the peptidoglycan hydrolase PcsB and FtsX and demonstrate that this interaction is essential for cell viability. As such, FtsX represents an attractive, conserved target for the development of new classes of antibiotics.

Structure of the Large Extracellular Loop of FtsX and Its Interaction with the Essential Peptidoglycan Hydrolase PcsB in Streptococcus pneumoniae.,Rued BE, Alcorlo M, Edmonds KA, Martinez-Caballero S, Straume D, Fu Y, Bruce KE, Wu H, Havarstein LS, Hermoso JA, Winkler ME, Giedroc DP MBio. 2019 Jan 29;10(1). pii: mBio.02622-18. doi: 10.1128/mBio.02622-18. PMID:30696736<ref>PMID:30696736</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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==See Also==
*[[Cell division protein 3D structures|Cell division protein 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Streptococcus pneumoniae D39]]
[[Category: Bruce KE]]
[[Category: Edmonds KA]]
[[Category: Fu Y]]
[[Category: Giedroc DP]]
[[Category: Rued BE]]
[[Category: Winkler ME]]
[[Category: Wu H]]