View source for 6mus


==Cryo-EM structure of larger Csm-crRNA-target RNA ternary complex in type III-A CRISPR-Cas system==
<SX load='6mus' size='340' side='right' viewer='molstar' caption='[[6mus]], [[Resolution|resolution]] 3.60&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6mus]] is a 10 chain structure with sequence from [http://en.wikipedia.org/wiki/"thermococcus_onnurineus"_bae_et_al._2006 "thermococcus onnurineus" bae et al. 2006]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MUS OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6MUS FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6mur|6mur]], [[6mua|6mua]]</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">TON_0893 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=342948 "Thermococcus onnurineus" Bae et al. 2006]), TON_0894 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=342948 "Thermococcus onnurineus" Bae et al. 2006]), TON_0895 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=342948 "Thermococcus onnurineus" Bae et al. 2006]), TON_0896 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=342948 "Thermococcus onnurineus" Bae et al. 2006]), TON_0897 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=342948 "Thermococcus onnurineus" Bae et al. 2006])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6mus FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mus OCA], [http://pdbe.org/6mus PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6mus RCSB], [http://www.ebi.ac.uk/pdbsum/6mus PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6mus ProSAT]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
Type IotaIotaIota CRISPR-Cas systems provide robust immunity against foreign RNA and DNA by sequence-specific RNase and target RNA-activated sequence-nonspecific DNase and RNase activities. We report on cryo-EM structures of Thermococcus onnurineus Csm(crRNA) binary, Csm(crRNA)-target RNA and Csm(crRNA)-target RNA(anti-tag) ternary complexes in the 3.1 A range. The topological features of the crRNA 5'-repeat tag explains the 5'-ruler mechanism for defining target cleavage sites, with accessibility of positions -2 to -5 within the 5'-repeat serving as sensors for avoidance of autoimmunity. The Csm3 thumb elements introduce periodic kinks in the crRNA-target RNA duplex, facilitating cleavage of the target RNA with 6-nt periodicity. Key Glu residues within a Csm1 loop segment of Csm(crRNA) adopt a proposed autoinhibitory conformation suggestive of DNase activity regulation. These structural findings, complemented by mutational studies of key intermolecular contacts, provide insights into Csm(crRNA) complex assembly, mechanisms underlying RNA targeting and site-specific periodic cleavage, regulation of DNase cleavage activity, and autoimmunity suppression.

Type III-A CRISPR-Cas Csm Complexes: Assembly, Periodic RNA Cleavage, DNase Activity Regulation, and Autoimmunity.,Jia N, Mo CY, Wang C, Eng ET, Marraffini LA, Patel DJ Mol Cell. 2018 Nov 27. pii: S1097-2765(18)30977-8. doi:, 10.1016/j.molcel.2018.11.007. PMID:30503773<ref>PMID:30503773</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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== References ==
<references/>
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[[Category: Thermococcus onnurineus bae et al. 2006]]
[[Category: Large Structures]]
[[Category: Eng, E T]]
[[Category: Jia, N]]
[[Category: Wang, C]]
[[Category: Cryo-em structure]]
[[Category: Larger csm-crrna-target rna ternary complex]]
[[Category: Rna binding protein-rna complex]]
[[Category: Type iii crispr-cas systerm]]