View source for 6mwx


==CryoEM structure of Chimeric Eastern Equine Encephalitis Virus with Fab of EEEV-69 Antibody==
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== Structural highlights ==
<table><tr><td colspan='2'>[[6mwx]] is a 16 chain structure with sequence from [https://en.wikipedia.org/wiki/Eastern_equine_encephalitis_virus Eastern equine encephalitis virus] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6MWX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6MWX FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 8.2&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6mwx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6mwx OCA], [https://pdbe.org/6mwx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6mwx RCSB], [https://www.ebi.ac.uk/pdbsum/6mwx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6mwx ProSAT]</span></td></tr>
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== Publication Abstract from PubMed ==
Alphaviruses are enveloped pathogens that cause arthritis and encephalitis. Here, we report a 4.4-A cryoelectron microscopy (cryo-EM) structure of eastern equine encephalitis virus (EEEV), an alphavirus that causes fatal encephalitis in humans. Our analysis provides insights into viral entry into host cells. The envelope protein E2 showed a binding site for the cellular attachment factor heparan sulfate. The presence of a cryptic E2 glycan suggests how EEEV escapes surveillance by lectin-expressing myeloid lineage cells, which are sentinels of the immune system. A mechanism for nucleocapsid core release and disassembly upon viral entry was inferred based on pH changes and capsid dissociation from envelope proteins. The EEEV capsid structure showed a viral RNA genome binding site adjacent to a ribosome binding site for viral genome translation following genome release. Using five Fab-EEEV complexes derived from neutralizing antibodies, our investigation provides insights into EEEV host cell interactions and protective epitopes relevant to vaccine design.

Cryo-EM Structures of Eastern Equine Encephalitis Virus Reveal Mechanisms of Virus Disassembly and Antibody Neutralization.,Hasan SS, Sun C, Kim AS, Watanabe Y, Chen CL, Klose T, Buda G, Crispin M, Diamond MS, Klimstra WB, Rossmann MG Cell Rep. 2018 Dec 11;25(11):3136-3147.e5. doi: 10.1016/j.celrep.2018.11.067. PMID:30540945<ref>PMID:30540945</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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==See Also==
*[[Antibody 3D structures|Antibody 3D structures]]
*[[Virus coat proteins 3D structures|Virus coat proteins 3D structures]]
*[[3D structures of non-human antibody|3D structures of non-human antibody]]
== References ==
<references/>
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[[Category: Eastern equine encephalitis virus]]
[[Category: Large Structures]]
[[Category: Mus musculus]]
[[Category: Buda G]]
[[Category: Chen CL]]
[[Category: Crispin M]]
[[Category: Diamond MS]]
[[Category: Hasan SS]]
[[Category: Kim AS]]
[[Category: Klimstra WB]]
[[Category: Klose T]]
[[Category: Rossmann MG]]
[[Category: Sun C]]
[[Category: Watanabe Y]]