View source for 6nbx


==T.elongatus NDH (data-set 2)==
<SX load='6nbx' size='340' side='right' viewer='molstar' caption='[[6nbx]], [[Resolution|resolution]] 3.50&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6nbx]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermosynechococcus_vestitus_BP-1 Thermosynechococcus vestitus BP-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NBX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6NBX FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.5&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6nbx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nbx OCA], [https://pdbe.org/6nbx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6nbx RCSB], [https://www.ebi.ac.uk/pdbsum/6nbx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6nbx ProSAT]</span></td></tr>
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== Function ==
[https://www.uniprot.org/uniprot/NU1C_THEVB NU1C_THEVB] NDH-1 shuttles electrons from an unknown electron donor, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory and/or the photosynthetic chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone. Couples the redox reaction to proton translocation, and thus conserves the redox energy in a proton gradient.[HAMAP-Rule:MF_01350]
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== Publication Abstract from PubMed ==
Cyclic electron flow around photosystem I (PSI) is a mechanism by which photosynthetic organisms balance the levels of ATP and NADPH necessary for efficient photosynthesis(1,2). NAD(P)H dehydrogenase-like complex (NDH) is a key component of this pathway in most oxygenic photosynthetic organisms(3,4) and is the last large photosynthetic membrane-protein complex for which the structure remains unknown. Related to the respiratory NADH dehydrogenase complex (complex I), NDH transfers electrons originating from PSI to the plastoquinone pool while pumping protons across the thylakoid membrane, thereby increasing the amount of ATP produced per NADP(+) molecule reduced(4,5). NDH possesses 11 of the 14 core complex I subunits, as well as several oxygenic-photosynthesis-specific (OPS) subunits that are conserved from cyanobacteria to plants(3,6). However, the three core complex I subunits that are involved in accepting electrons from NAD(P)H are notably absent in NDH(3,5,6), and it is therefore not clear how NDH acquires and transfers electrons to plastoquinone. It is proposed that the OPS subunits-specifically NdhS-enable NDH to accept electrons from its electron donor, ferredoxin(3-5,7). Here we report a 3.1 A structure of the 0.42-MDa NDH complex from the thermophilic cyanobacterium Thermosynechococcus elongatus BP-1, obtained by single-particle cryo-electron microscopy. Our maps reveal the structure and arrangement of the principal OPS subunits in the NDH complex, as well as an unexpected cofactor close to the plastoquinone-binding site in the peripheral arm. The location of the OPS subunits supports a role in electron transfer and defines two potential ferredoxin-binding sites at the apex of the peripheral arm. These results suggest that NDH could possess several electron transfer routes, which would serve to maximize plastoquinone reduction and avoid deleterious off-target chemistry of the semi-plastoquinone radical.

Structure of the complex I-like molecule NDH of oxygenic photosynthesis.,Laughlin TG, Bayne AN, Trempe JF, Savage DF, Davies KM Nature. 2019 Feb;566(7744):411-414. doi: 10.1038/s41586-019-0921-0. Epub 2019 Feb, 11. PMID:30742075<ref>PMID:30742075</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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==See Also==
*[[NADH-quinone oxidoreductase|NADH-quinone oxidoreductase]]
== References ==
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[[Category: Large Structures]]
[[Category: Thermosynechococcus vestitus BP-1]]
[[Category: Bayne A]]
[[Category: Davies KM]]
[[Category: Laughlin TG]]
[[Category: Savage DF]]
[[Category: Trempe J-F]]