View source for 6ncm


==Crystal structure of the human FOXN3 DNA binding domain in complex with a forkhead-like (FHL) DNA sequence==
<StructureSection load='6ncm' size='340' side='right'caption='[[6ncm]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6ncm]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NCM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6NCM FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.704&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ncm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ncm OCA], [https://pdbe.org/6ncm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ncm RCSB], [https://www.ebi.ac.uk/pdbsum/6ncm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ncm ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/FOXN3_HUMAN FOXN3_HUMAN] Acts as a transcriptional repressor. May be involved in DNA damage-inducible cell cycle arrests (checkpoints).<ref>PMID:16102918</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Transcription factors (TFs) control gene expression by binding DNA recognition sites in genomic regulatory regions. Although most forkhead TFs recognize a canonical forkhead (FKH) motif, RYAAAYA, some forkheads recognize a completely different (FHL) motif, GACGC. Bispecific forkhead proteins recognize both motifs, but the molecular basis for bispecific DNA recognition is not understood. We present co-crystal structures of the FoxN3 DNA binding domain bound to the FKH and FHL sites, respectively. FoxN3 adopts a similar conformation to recognize both motifs, making contacts with different DNA bases using the same amino acids. However, the DNA structure is different in the two complexes. These structures reveal how a single TF binds two unrelated DNA sequences and the importance of DNA shape in the mechanism of bispecific recognition.

Bispecific Forkhead Transcription Factor FoxN3 Recognizes Two Distinct Motifs with Different DNA Shapes.,Rogers JM, Waters CT, Seegar TCM, Jarrett SM, Hallworth AN, Blacklow SC, Bulyk ML Mol Cell. 2019 Feb 22. pii: S1097-2765(19)30039-5. doi:, 10.1016/j.molcel.2019.01.019. PMID:30826165<ref>PMID:30826165</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6ncm" style="background-color:#fffaf0;"></div>

==See Also==
*[[FOX 3D structures|FOX 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Synthetic construct]]
[[Category: Blacklow SC]]
[[Category: Bulyk ML]]
[[Category: Hallworth AN]]
[[Category: Jarrett SM]]
[[Category: Rogers JM]]
[[Category: Seegar TC]]
[[Category: Waters CT]]