View source for 6nhh


==Rhodobacter sphaeroides bc1 with azoxystrobin==
<StructureSection load='6nhh' size='340' side='right'caption='[[6nhh]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6nhh]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Cereibacter_sphaeroides_2.4.1 Cereibacter sphaeroides 2.4.1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NHH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6NHH FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=6PE:1,2-DIHEXANOYL-SN-GLYCERO-3-PHOSPHOETHANOLAMINE'>6PE</scene>, <scene name='pdbligand=8SP:O-[(R)-{[(2R)-2,3-BIS(OCTANOYLOXY)PROPYL]OXY}(HYDROXY)PHOSPHORYL]-L-SERINE'>8SP</scene>, <scene name='pdbligand=AZO:METHYL+(2Z)-2-(2-{[6-(2-CYANOPHENOXY)PYRIMIDIN-4-YL]OXY}PHENYL)-3-METHOXYACRYLATE'>AZO</scene>, <scene name='pdbligand=BOG:B-OCTYLGLUCOSIDE'>BOG</scene>, <scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=HEC:HEME+C'>HEC</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=SR:STRONTIUM+ION'>SR</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6nhh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nhh OCA], [https://pdbe.org/6nhh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6nhh RCSB], [https://www.ebi.ac.uk/pdbsum/6nhh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6nhh ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q3IY10_CERS4 Q3IY10_CERS4] Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis.[ARBA:ARBA00002444][RuleBase:RU003385]
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== Publication Abstract from PubMed ==
Cytochrome bc1 complexes (cyt bc1), also known as Complex III in mitochondria, are components of the cellular respiratory chain and of the photosynthetic apparatus of nonoxygeneic photosynthetic bacteria. They catalyze electron transfer (ET) from ubiquinol to cytochrome c and concomitantly translocate protons across the membrane, contributing to the cross-membrane potential essential for a myriad of cellular activities. This ET-coupled proton translocation reaction requires a gating mechanism that ensures bifurcated electron flow. Here, we report the observation of the Rieske iron-sulfur protein (ISP) in a mobile state, as revealed by the crystal structure of cyt bc1 from the photosynthetic bacterium Rhodobacter sphaeroides in complex with the fungicide azoxystrobin. Unlike cyt bc1 inhibitors stigmatellin and famoxadone that immobilize the ISP, azoxystrobin causes the ISP to separate from the cyt b subunit and to remain in a mobile state. Analysis of anomalous scattering signals from the iron-sulfur cluster of the ISP suggests the existence of a trajectory for electron delivery. This work supports and solidifies the hypothesis that the bimodal conformation switch of the ISP provides a gating mechanism for bifurcated ET, which is essential to the Qcycle mechanism of cyt bc1 function.

The Crystal Structure of Bacterial Cytochrome bc1 in Complex with Azoxystrobin Reveals a Conformation Switch of the Rieske Iron-Sulfur Protein Subunit.,Esser L, Zhou F, Yu CA, Xia D J Biol Chem. 2019 Jun 10. pii: RA119.008381. doi: 10.1074/jbc.RA119.008381. PMID:31182483<ref>PMID:31182483</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 6nhh" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Cereibacter sphaeroides 2 4.1]]
[[Category: Large Structures]]
[[Category: Xia D]]
[[Category: Yu CA]]
[[Category: Zhou F]]