View source for 6nii


==Crystal structure of RavD (residues 1-200) from Legionella pneumophila (strain Corby)==
<StructureSection load='6nii' size='340' side='right'caption='[[6nii]], [[Resolution|resolution]] 2.15&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6nii]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Legionella_pneumophila Legionella pneumophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NII OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6NII FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.15&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6nii FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nii OCA], [https://pdbe.org/6nii PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6nii RCSB], [https://www.ebi.ac.uk/pdbsum/6nii PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6nii ProSAT]</span></td></tr>
</table>
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== Publication Abstract from PubMed ==
Linear ubiquitin (Ub) chains regulate many cellular processes, including NF-kappaB immune signalling. Pathogenic bacteria have evolved to secrete effector proteins that harbour deubiquitinase activity into host cells to disrupt host ubiquitination signalling. All previously identified effector deubiquitinases hydrolyse isopeptide-linked polyubiquitin (polyUb). It has been a long-standing question whether bacterial pathogens have evolved an effector deubiquitinase to directly cleave linear Ub chains. In this study, we performed extensive screening of bacterial pathogens and found that Legionella pneumophila-the causative agent of human Legionnaire's disease-encodes an effector protein, RavD, which harbours deubiquitinase activity exquisitely specific for linear Ub chains. RavD hydrolyses linear Ub chains but not any type of isopeptide-linked polyUb. The crystal structure of RavD with linear diubiquitin reveals that RavD adopts a papain-like fold with a Cys-His-Ser catalytic triad. The Ub-binding surface and specific interacting residues in RavD determine its specificity for Met1 linkages. RavD prevents the accumulation of linear Ub chains on Legionella-containing vacuoles established by the pathogen in host cells to inhibit the NF-kappaB pathway during infection. This study identified a unique linear Ub chain-specific effector deubiquitinase and indicates its potential application as a tool to dissect linear polyUb-mediated signalling in mammalian cells.

A bacterial effector deubiquitinase specifically hydrolyses linear ubiquitin chains to inhibit host inflammatory signalling.,Wan M, Wang X, Huang C, Xu D, Wang Z, Zhou Y, Zhu Y Nat Microbiol. 2019 May 20. pii: 10.1038/s41564-019-0454-1. doi:, 10.1038/s41564-019-0454-1. PMID:31110362<ref>PMID:31110362</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 6nii" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Large Structures]]
[[Category: Legionella pneumophila]]
[[Category: Wang X]]
[[Category: Zhou Y]]
[[Category: Zhu Y]]