View source for 6niv


==Racemic Phenol-Soluble Modulin Alpha 3 Peptide==
<StructureSection load='6niv' size='340' side='right'caption='[[6niv]], [[Resolution|resolution]] 1.45&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6niv]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NIV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6NIV FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.45&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6niv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6niv OCA], [https://pdbe.org/6niv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6niv RCSB], [https://www.ebi.ac.uk/pdbsum/6niv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6niv ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/H9BRQ7_STAAU H9BRQ7_STAAU] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Phenol-soluble modulin alpha3 (PSMalpha3) is a cytotoxic peptide secreted by virulent strains of Staphylococcus aureus. We used a stereochemical strategy to examine the mechanism of PSMalpha3-mediated toxicity. One hypothesis is that PSMalpha3 toxicity requires fibril formation; an alternative is that toxicity is caused by soluble forms of PSMalpha3, possibly oligomeric. We find that the unnatural enantiomer (D residues) displays cytotoxicity comparable to that of L-PSMalpha3. Racemic PSMalpha3 is similarly toxic to enantiopure PSMalpha3 (L or D) under some conditions, but the toxicity is lost under conditions that cause racemic PSMalpha3 to aggregate. A crystal structure of racemic PSMalpha3-NH2 displays an alpha-helical secondary structure and a packing pattern that is reminiscent of the cross-alpha arrangement recently discovered in crystals of L-PSMalpha3. Our data suggest that the cytotoxicity of PSMalpha3 does not depend on stereospecific engagement of a target protein or other chiral macromolecule, an observation that supports a mechanism based on membrane disruption. In addition, our data support the hypothesis that toxicity is exerted by a soluble form rather than an insoluble fibrillar form.

Use of a Stereochemical Strategy To Probe the Mechanism of Phenol-Soluble Modulin alpha3 Toxicity.,Yao Z, Cary BP, Bingman CA, Wang C, Kreitler DF, Satyshur KA, Forest KT, Gellman SH J Am Chem Soc. 2019 May 15;141(19):7660-7664. doi: 10.1021/jacs.9b00349. Epub, 2019 May 2. PMID:31045358<ref>PMID:31045358</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6niv" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Staphylococcus aureus]]
[[Category: Bingman CA]]
[[Category: Cary BP]]
[[Category: Forest KT]]
[[Category: Gellman SH]]
[[Category: Kreitler DF]]
[[Category: Satyshur KA]]
[[Category: Wang C]]
[[Category: Yao Z]]