View source for 6nop


==Structure of Cyanothece McdA(D38A)-ATP complex==
<StructureSection load='6nop' size='340' side='right'caption='[[6nop]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6nop]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Cyanothece Cyanothece]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NOP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6NOP FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6nop FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6nop OCA], [https://pdbe.org/6nop PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6nop RCSB], [https://www.ebi.ac.uk/pdbsum/6nop PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6nop ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/B7KMS4_GLOC7 B7KMS4_GLOC7] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Carboxysomes, protein-coated organelles in cyanobacteria, are important in global carbon fixation. However, these organelles are present at low copy in each cell and hence must be segregated to ensure transmission from one generation to the next. Recent studies revealed that a DNA partition-like ParA-ParB system mediates carboxysome maintenance, called McdA-McdB. Here, we describe the first McdA and McdB homolog structures. McdA is similar to partition ParA Walker-box proteins, but lacks the P-loop signature lysine involved in ATP binding. Strikingly, a McdA-ATP structure shows that a lysine distant from the P-loop and conserved in McdA homologs, enables ATP-dependent nucleotide sandwich dimer formation. Similar to partition ParA proteins this ATP-bound form binds nonspecific-DNA. McdB, which we show directly binds McdA, harbors a unique fold and appears to form higher-order oligomers like partition ParB proteins. Thus, our data reveal a new signature motif that enables McdA dimer formation and indicates that, similar to DNA segregation, carboxysome maintenance systems employ Walker-box proteins as DNA-binding motors while McdB proteins form higher order oligomers, which could function as adaptors to link carboxysomes and provide for stable transport by the McdA proteins.

Structures of maintenance of carboxysome distribution Walker-box McdA and McdB adaptor homologs.,Schumacher MA, Henderson M, Zhang H Nucleic Acids Res. 2019 May 20. pii: 5491742. doi: 10.1093/nar/gkz314. PMID:31106331<ref>PMID:31106331</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6nop" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Cyanothece]]
[[Category: Large Structures]]
[[Category: Schumacher MA]]