View source for 6oaw


==Crystal structure of a CRISPR Cas-related protein==
<StructureSection load='6oaw' size='340' side='right'caption='[[6oaw]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6oaw]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Ruminococcus_sp. Ruminococcus sp.]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6OAW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6OAW FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6oaw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6oaw OCA], [https://pdbe.org/6oaw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6oaw RCSB], [https://www.ebi.ac.uk/pdbsum/6oaw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6oaw ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The Type VI-D CRISPR-Cas system employs an RNA-guided RNase Cas13d with minimal targeting constraints to combat viral infections. This CRISPR system contains RspWYL1 as a unique accessory protein that plays a key role in boosting its effector function on target RNAs, but the mechanism behind this RspWYL1-mediated stimulation remains completely unexplored. Through structural and biophysical approaches, we reveal that the full-length RspWYL1 possesses a novel three-domain architecture and preferentially binds ssRNA with high affinity. Specifically, the N-terminus of RspWYL1 harbors a ribbon-helix-helix motif reminiscent of transcriptional regulators; the central WYL domain of RspWYL1 displays a Sm-like beta-barrel fold; and the C-terminal domain of RspWYL1 primarily contributes to the dimerization of RspWYL1 and may regulate the RspWYL1 function via a large conformational change. Collectively, this study provides a first glimpse into the complex mechanism behind the RspWYL1-dictated boosting of target ssRNA cleavage in the Type VI-D CRISPR-Cas system.

Structural insights into the modulatory role of the accessory protein WYL1 in the Type VI-D CRISPR-Cas system.,Zhang H, Dong C, Li L, Wasney GA, Min J Nucleic Acids Res. 2019 Apr 12. pii: 5446253. doi: 10.1093/nar/gkz269. PMID:30976796<ref>PMID:30976796</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6oaw" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Ruminococcus sp]]
[[Category: Arrowsmith CH]]
[[Category: Bountra C]]
[[Category: Dong C]]
[[Category: Edwards AM]]
[[Category: Li L]]
[[Category: Min J]]
[[Category: Structural genomic]]
[[Category: Tempel W]]
[[Category: Zhang H]]