View source for 6obt


==Structural insights into dehydratase substrate selection for the borrelidin and fluvirucin polyketide synthases==
<StructureSection load='6obt' size='340' side='right'caption='[[6obt]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6obt]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_parvulus Streptomyces parvulus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6OBT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6OBT FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6obt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6obt OCA], [https://pdbe.org/6obt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6obt RCSB], [https://www.ebi.ac.uk/pdbsum/6obt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6obt ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q70I00_9ACTN Q70I00_9ACTN] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Engineered polyketide synthases (PKSs) are promising synthetic biology platforms for the production of chemicals with diverse applications. The dehydratase (DH) domain within modular type I PKSs generates an alpha,beta-unsaturated bond in nascent polyketide intermediates through a dehydration reaction. Several crystal structures of DH domains have been solved, providing important structural insights into substrate selection and dehydration. Here, we present two DH domain structures from two chemically diverse PKSs. The first DH domain, isolated from the third module in the borrelidin PKS, is specific towards a trans-cyclopentane-carboxylate-containing polyketide substrate. The second DH domain, isolated from the first module in the fluvirucin B1 PKS, accepts an amide-containing polyketide intermediate. Sequence-structure analysis of these domains, in addition to previously published DH structures, display many significant similarities and key differences pertaining to substrate selection. The two major differences between BorA DH M3, FluA DH M1 and other DH domains are found in regions of unmodeled residues or residues containing high B-factors. These two regions are located between alpha3-beta11 and beta7-alpha2. From the catalytic Asp located in alpha3 to a conserved Pro in beta11, the residues between them form part of the bottom of the substrate-binding cavity responsible for binding to acyl-ACP intermediates.

Structural insights into dehydratase substrate selection for the borrelidin and fluvirucin polyketide synthases.,Barajas JF, McAndrew RP, Thompson MG, Backman TWH, Pang B, de Rond T, Pereira JH, Benites VT, Martin HG, Baidoo EEK, Hillson NJ, Adams PD, Keasling JD J Ind Microbiol Biotechnol. 2019 May 21. pii: 10.1007/s10295-019-02189-z. doi:, 10.1007/s10295-019-02189-z. PMID:31115703<ref>PMID:31115703</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6obt" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Streptomyces parvulus]]
[[Category: Adams PD]]
[[Category: Barajas JF]]
[[Category: Keasling JD]]
[[Category: McAndrew RP]]
[[Category: Pereira JH]]