View source for 6oj3


==In situ structure of rotavirus VP1 RNA-dependent RNA polymerase (TLP)==
<SX load='6oj3' size='340' side='right' viewer='molstar' caption='[[6oj3]], [[Resolution|resolution]] 4.50&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6oj3]] is a 11 chain structure with sequence from [https://en.wikipedia.org/wiki/Simian_rotavirus_A_strain_RRV Simian rotavirus A strain RRV]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6OJ3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6OJ3 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.5&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6oj3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6oj3 OCA], [https://pdbe.org/6oj3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6oj3 RCSB], [https://www.ebi.ac.uk/pdbsum/6oj3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6oj3 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/B3F2X3_ROTRH B3F2X3_ROTRH] Inner capsid protein that self-assembles to form an icosahedral capsid with a T=2 symmetry, which consists of 120 copies of VP2, with channels at each of its five-fold vertices. This capsid constitutes the innermost concentric layer of the viral mature particle. It encapsidates the polymerase VP1, the capping enzyme VP3 and the genomic dsRNA, thereby defining the core. The innermost VP2 capsid and the intermediate VP6 capsid remain intact following cell entry to protect the dsRNA from degradation and to prevent unfavorable antiviral responses in the host cell during all the replication cycle of the virus. Nascent transcripts are transcribed within the structural confines of this double-layered particle (DLP) and are extruded through the channels formed by VP2 N-termini. VP2 is required for the replicase activity of VP1 polymerase. Probably recruits a copy of a VP1-VP3 complex, potentially along with a segment of plus-strand RNA, as a decamer of VP2 assembles. May activate the autoinhibited VP1/RNA complex to coordinate packaging and genome replication.[RuleBase:RU363125]
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[[Category: Large Structures]]
[[Category: Simian rotavirus A strain RRV]]
[[Category: Estrozi LF]]
[[Category: Grant T]]
[[Category: Grigorieff N]]
[[Category: Harrison SC]]
[[Category: Herrmann T]]
[[Category: Jenni S]]
[[Category: Li Z]]
[[Category: Salgado EN]]
[[Category: Trapani S]]