View source for 6onp


==Crystal structure of periplasmic binding protein XoxJ from Methylobacterium extorquens AM1==
<StructureSection load='6onp' size='340' side='right'caption='[[6onp]], [[Resolution|resolution]] 2.27&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6onp]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Methylorubrum_extorquens_AM1 Methylorubrum extorquens AM1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ONP OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6ONP FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.27&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6onp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6onp OCA], [https://pdbe.org/6onp PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6onp RCSB], [https://www.ebi.ac.uk/pdbsum/6onp PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6onp ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/C5B122_METEA C5B122_METEA] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Lanthanide (Ln)-dependent methanol dehydrogenases (MDHs) have been recently shown to be widespread in methylotrophic bacteria. Along with the core MDH protein, XoxF, these systems comprise two other proteins, XoxG (a c-type cytochrome) and XoxJ (a periplasmic binding protein of unknown function), about which little is known. Here, we biochemically and structurally characterize these proteins from the methyltroph, Methylobacterium extorquens AM1. In contrast to results obtained via an artificial assay system, assays of XoxFs metallated with LaIII, CeIII, and NdIII using their physiological electron acceptor, XoxG, display Ln-independent activities, but the Km for XoxG markedly increases from La to Nd. This result suggests that XoxG's redox properties are tuned specifically for lighter Lns in XoxF, an interpretation supported by the unusually low reduction potential of XoxG (+172 mV). The x-ray crystal structure of XoxG provides a structural basis for this reduction potential and insight into the XoxG-XoxF interaction. Finally, the x-ray crystal structure of XoxJ reveals a large hydrophobic cleft and suggests a role in activation of XoxF. These studies enrich our understanding of the underlying chemical principles that enable the activity of XoxF with multiple lanthanides in vitro and in vivo.

Biochemical and structural characterization of XoxG and XoxJ and their roles in activity of the lanthanide-dependent methanol dehydrogenase, XoxF.,Featherston ER, Rose HR, McBride MJ, Taylor E, Boal AK, Cotruvo J Jr Chembiochem. 2019 Apr 24. doi: 10.1002/cbic.201900184. PMID:31017712<ref>PMID:31017712</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6onp" style="background-color:#fffaf0;"></div>

==See Also==
*[[Methanol dehydrogenase|Methanol dehydrogenase]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Methylorubrum extorquens AM1]]
[[Category: Boal AK]]
[[Category: Rose HR]]
[[Category: Taylor EM]]