View source for 6ooc


==Structure of the pterocarpan synthase dirigent protein GePTS1==
<StructureSection load='6ooc' size='340' side='right'caption='[[6ooc]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6ooc]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Glycyrrhiza_echinata Glycyrrhiza echinata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6OOC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6OOC FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ooc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ooc OCA], [https://pdbe.org/6ooc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ooc RCSB], [https://www.ebi.ac.uk/pdbsum/6ooc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ooc ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/DIR_GLYEC DIR_GLYEC] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The biochemical activities of dirigent proteins (DPs) give rise to distinct complex classes of plant phenolics. DPs apparently began to emerge during the aquatic-to-land transition, with phylogenetic analyses revealing the presence of numerous DP subfamilies in the plant kingdom. The vast majority (&gt;95%) of DPs in these large multigene families still await discovery of their biochemical functions. Here, we elucidated the 3D structures of two pterocarpan-forming proteins with dirigent-like domains. Both proteins stereospecifically convert distinct diastereomeric chiral isoflavonoid precursors to the chiral pterocarpans, (-)- and (+)-medicarpin, respectively. Their 3D structures enabled comparisons with stereoselective lignan- and aromatic terpenoid-forming DP orthologs. Each protein provides entry into diverse plant natural products classes, and our experiments suggest a common biochemical mechanism in binding and stabilizing distinct plant phenol-derived mono- and bis-quinone methide intermediates during different C-C and C-O bond-forming processes. These observations provide key insights into both their appearance and functional diversification of DPs during land plant evolution/adaptation. The proposed biochemical mechanisms based on our findings provide important clues to how additional physiological roles for DPs and proteins harboring dirigent-like domains can now be rationally and systematically identified.

Pterocarpan synthase (PTS) structures suggest a common quinone methide-stabilizing function in dirigent proteins and proteins with dirigent-like domains.,Meng Q, Moinuddin SGA, Kim SJ, Bedgar DL, Costa MA, Thomas DG, Young RP, Smith CA, Cort JR, Davin LB, Lewis NG J Biol Chem. 2020 Aug 14;295(33):11584-11601. doi: 10.1074/jbc.RA120.012444. Epub, 2020 Jun 21. PMID:32565424<ref>PMID:32565424</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6ooc" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Glycyrrhiza echinata]]
[[Category: Large Structures]]
[[Category: Smith CA]]