View source for 6p0f


==N-terminal domain of Thermococcus Gammatolerans McrB==
<StructureSection load='6p0f' size='340' side='right'caption='[[6p0f]], [[Resolution|resolution]] 1.68&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6p0f]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermococcus_gammatolerans_EJ3 Thermococcus gammatolerans EJ3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6P0F OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6P0F FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.683&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=NH4:AMMONIUM+ION'>NH4</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6p0f FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6p0f OCA], [https://pdbe.org/6p0f PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6p0f RCSB], [https://www.ebi.ac.uk/pdbsum/6p0f PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6p0f ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/C5A3Z3_THEGJ C5A3Z3_THEGJ] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
McrBC is a two-component, modification-dependent restriction system that cleaves foreign DNA containing methylated cytosines. Previous crystallographic studies have shown that Escherichia coli McrB uses a base-flipping mechanism to recognize these modified substrates with high affinity. The sidechains stabilizing both the flipped base and the distorted duplex are poorly conserved among McrB homologs, suggesting that other mechanisms may exist for binding modified DNA. Here we present the structures of the Thermococcus gammatolerans McrB DNA binding domain (Tg185) both alone and in complex with a methylated DNA substrate at 1.68 A and 2.27 A resolution respectively. The structures reveal that Tg185 consists of a YT521-B homology (YTH) domain, which is commonly found in eukaryotic proteins that bind methylated RNA and is structurally unrelated to the E. coli McrB DNA binding domain. Structural superposition and co-crystallization further show that Tg185 shares a conserved aromatic cage with other YTH domains, which forms the binding pocket for a flipped-out base. Mutational analysis of this aromatic cage supports its role in conferring specificity for the methylated adenines while an extended basic surface present in Tg185 facilitates its preferential binding to duplex DNA rather than RNA. Together these findings establish a new binding mode and specificity among McrB homologs and expand the biological roles of YTH domains.

The structure of the Thermococcus gammatolerans McrB N-terminal domain reveals a new mode of substrate recognition and specificity among McrB homologs.,Hosford CJ, Bui AQ, Chappie JS J Biol Chem. 2019 Dec 10. pii: RA119.010188. doi: 10.1074/jbc.RA119.010188. PMID:31822563<ref>PMID:31822563</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6p0f" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</StructureSection>
[[Category: Large Structures]]
[[Category: Thermococcus gammatolerans EJ3]]
[[Category: Chappie JS]]
[[Category: Hosford CJ]]