View source for 6pbh


==Crystal Structure of HLA-A*68:01 in complex with NP145-156, a 12 mer influenza peptide==
<StructureSection load='6pbh' size='340' side='right'caption='[[6pbh]], [[Resolution|resolution]] 1.89&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6pbh]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Unidentified_influenza_virus Unidentified influenza virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6PBH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6PBH FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.89&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IOD:IODIDE+ION'>IOD</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6pbh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6pbh OCA], [https://pdbe.org/6pbh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6pbh RCSB], [https://www.ebi.ac.uk/pdbsum/6pbh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6pbh ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Although influenza viruses lead to severe illness in high-risk populations, host genetic factors associated with severe disease are largely unknown. As the HLA-A*68:01 allele can be linked to severe pandemic 2009-H1N1 disease, we investigate a potential impairment of HLA-A*68:01-restricted CD8(+) T cells to mount robust responses. We elucidate the HLA-A*68:01(+)CD8(+) T cell response directed toward an extended influenza-derived nucleoprotein (NP) peptide and show that only ~35% individuals have immunodominant A68/NP145(+)CD8(+) T cell responses. Dissecting A68/NP145(+)CD8(+) T cells in low vs. medium/high responders reveals that high responding donors have A68/NP145(+)CD8(+) memory T cells with clonally expanded TCRalphabetas, while low-responders display A68/NP145(+)CD8(+) T cells with predominantly naive phenotypes and non-expanded TCRalphabetas. Single-cell index sorting and TCRalphabeta analyses link expansion of A68/NP145(+)CD8(+) T cells to their memory potential. Our study demonstrates the immunodominance potential of influenza-specific CD8(+) T cells presented by a risk HLA-A*68:01 molecule and advocates for priming CD8(+) T cell compartments in HLA-A*68:01-expressing individuals for establishment of pre-existing protective memory T cell pools.

Challenging immunodominance of influenza-specific CD8(+) T cell responses restricted by the risk-associated HLA-A*68:01 allomorph.,van de Sandt CE, Clemens EB, Grant EJ, Rowntree LC, Sant S, Halim H, Crowe J, Cheng AC, Kotsimbos TC, Richards M, Miller A, Tong SYC, Rossjohn J, Nguyen THO, Gras S, Chen W, Kedzierska K Nat Commun. 2019 Dec 6;10(1):5579. doi: 10.1038/s41467-019-13346-4. PMID:31811120<ref>PMID:31811120</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6pbh" style="background-color:#fffaf0;"></div>

==See Also==
*[[Beta-2 microglobulin 3D structures|Beta-2 microglobulin 3D structures]]
*[[MHC 3D structures|MHC 3D structures]]
*[[MHC I 3D structures|MHC I 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Unidentified influenza virus]]
[[Category: Gras S]]