View source for 6pii


==The evolving story of AtzT, a periplasmic binding protein==
<StructureSection load='6pii' size='340' side='right'caption='[[6pii]], [[Resolution|resolution]] 1.87&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6pii]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_sp._ADP Pseudomonas sp. ADP]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6PII OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6PII FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.87&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EMC:ETHYL+MERCURY+ION'>EMC</scene>, <scene name='pdbligand=GUN:GUANINE'>GUN</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6pii FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6pii OCA], [https://pdbe.org/6pii PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6pii RCSB], [https://www.ebi.ac.uk/pdbsum/6pii PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6pii ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q936X6_PSESD Q936X6_PSESD] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Atrazine is an s-triazine-based herbicide that is used in many countries around the world in many millions of tons per year. A small number of organisms, such as Pseudomonas sp. strain ADP, have evolved to use this modified s-triazine as a food source, and the various genes required to metabolize atrazine can be found on a single plasmid. The atomic structures of seven of the eight proteins involved in the breakdown of atrazine by Pseudomonas sp. strain ADP have been determined by X-ray crystallography, but the structures of the proteins required by the cell to import atrazine for use as an energy source are still lacking. The structure of AtzT, a periplasmic binding protein that may be involved in the transport of a derivative of atrazine, 2-hydroxyatrazine, into the cell for mineralization, has now been determined. The structure was determined by SAD phasing using an ethylmercury phosphate derivative that diffracted X-rays to beyond 1.9 A resolution. `Native' (guanine-bound) and 2-hydroxyatrazine-bound structures were also determined to high resolution (1.67 and 1.65 A, respectively), showing that 2-hydroxyatrazine binds in a similar way to the purportedly native ligand. Structural similarities led to the belief that it may be possible to evolve AtzT from a purine-binding protein to a protein that can bind and detect atrazine in the environment.

The evolving story of AtzT, a periplasmic binding protein.,Dennis ML, Esquirol L, Nebl T, Newman J, Scott C, Peat TS Acta Crystallogr D Struct Biol. 2019 Nov 1;75(Pt 11):995-1002. doi:, 10.1107/S2059798319013883. Epub 2019 Oct 31. PMID:31692473<ref>PMID:31692473</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6pii" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Pseudomonas sp. ADP]]
[[Category: Dennis M]]
[[Category: Esquirol L]]
[[Category: Nebl T]]
[[Category: Newman J]]
[[Category: Peat TS]]
[[Category: Scott C]]