View source for 6po6


==MicroED Structure of a Natural Product VFAThiaGlu==
<StructureSection load='6po6' size='340' side='right'caption='[[6po6]], [[Resolution|resolution]] 1.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6po6]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_syringae Pseudomonas syringae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6PO6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6PO6 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron crystallography, [[Resolution|Resolution]] 1&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=OV7:(2R)-amino[(carboxymethyl)sulfanyl]acetic+acid'>OV7</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6po6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6po6 OCA], [https://pdbe.org/6po6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6po6 RCSB], [https://www.ebi.ac.uk/pdbsum/6po6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6po6 ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Genome sequencing of environmental bacteria allows identification of biosynthetic gene clusters encoding unusual combinations of enzymes that produce unknown natural products. We identified a pathway in which a ribosomally synthesized small peptide serves as a scaffold for nonribosomal peptide extension and chemical modification. Amino acids are transferred to the carboxyl terminus of the peptide through adenosine triphosphate and amino acyl-tRNA-dependent chemistry that is independent of the ribosome. Oxidative rearrangement, carboxymethylation, and proteolysis of a terminal cysteine yields an amino acid-derived small molecule. Microcrystal electron diffraction demonstrates that the resulting product is isosteric to glutamate. We show that a similar peptide extension is used during the biosynthesis of the ammosamides, which are cytotoxic pyrroloquinoline alkaloids. These results suggest an alternative paradigm for biosynthesis of amino acid-derived natural products.

Use of a scaffold peptide in the biosynthesis of amino acid-derived natural products.,Ting CP, Funk MA, Halaby SL, Zhang Z, Gonen T, van der Donk WA Science. 2019 Jul 19;365(6450):280-284. doi: 10.1126/science.aau6232. PMID:31320540<ref>PMID:31320540</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6po6" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Pseudomonas syringae]]
[[Category: Funk MA]]
[[Category: Gonen T]]
[[Category: Halaby S]]
[[Category: Ting CP]]
[[Category: Van der Donk WA]]