View source for 6ptn


==Structure of Ctf4 trimer in complex with two CMG helicases==
<SX load='6ptn' size='340' side='right' viewer='molstar' caption='[[6ptn]], [[Resolution|resolution]] 5.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6ptn]] is a 21 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6PTN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6PTN FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 5.8&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ptn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ptn OCA], [https://pdbe.org/6ptn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ptn RCSB], [https://www.ebi.ac.uk/pdbsum/6ptn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ptn ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/CTF4_YEAST CTF4_YEAST] Accessory factor for DNA replication. It plays a role in accurately duplicating the genome in vivo.
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== Publication Abstract from PubMed ==
The current view is that eukaryotic replisomes are independent. Here we show that Ctf4 tightly dimerizes CMG helicase, with an extensive interface involving Psf2, Cdc45, and Sld5. Interestingly, Ctf4 binds only one Pol alpha-primase. Thus, Ctf4 may have evolved as a trimer to organize two helicases and one Pol alpha-primase into a replication factory. In the 2CMG-Ctf43-1Pol alpha-primase factory model, the two CMGs nearly face each other, placing the two lagging strands toward the center and two leading strands out the sides. The single Pol alpha-primase is centrally located and may prime both sister replisomes. The Ctf4-coupled-sister replisome model is consistent with cellular microscopy studies revealing two sister forks of an origin remain attached and are pushed forward from a protein platform. The replication factory model may facilitate parental nucleosome transfer during replication.

Ctf4 organizes sister replisomes and Pol alpha into a replication factory.,Yuan Z, Georgescu R, Santos RLA, Zhang D, Bai L, Yao NY, Zhao G, O'Donnell ME, Li H Elife. 2019 Oct 7;8. pii: 47405. doi: 10.7554/eLife.47405. PMID:31589141<ref>PMID:31589141</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 6ptn" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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</SX>
[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Bai L]]
[[Category: Donnell M]]
[[Category: Georgescu R]]
[[Category: Li H]]
[[Category: Santos R]]
[[Category: Yuan Z]]