View source for 6pxs


==Crystal structure of iminodiacetate oxidase (IdaA) from Chelativorans sp. BNC1==
<StructureSection load='6pxs' size='340' side='right'caption='[[6pxs]], [[Resolution|resolution]] 2.84&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6pxs]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Chelativorans_sp._BNC1 Chelativorans sp. BNC1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6PXS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6PXS FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.836&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6pxs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6pxs OCA], [https://pdbe.org/6pxs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6pxs RCSB], [https://www.ebi.ac.uk/pdbsum/6pxs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6pxs ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q11HA4_CHESB Q11HA4_CHESB] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Ethylenediaminetetraacetate (EDTA) is the most abundant organic pollutant in surface water because of its extensive usage and the recalcitrance of stable metal-EDTA complexes. A few bacteria including Chelativorans sp. BNC1 can degrade EDTA with a monooxygenase to ethylenediaminediacetate (EDDA) and then use iminodiacetate oxidase (IdaA) to further degrade EDDA into ethylenediamine in a two-step oxidation. To alleviate EDTA pollution into the environment, deciphering the mechanisms of the metabolizing enzymes is an imperative prerequisite for informed EDTA bioremediation. Although IdaA cannot oxidize glycine, the crystal structure of IdaA shows its tertiary and quaternary structures similar to those of glycine oxidases. All confirmed substrates, EDDA, ethylenediaminemonoacetate (EDMA), iminodiacetate, and sarcosine are secondary amines with at least one N-acetyl group. Each substrate was bound at the re-side face of the isoalloxazine ring in a solvent-connected cavity. The carboxyl group of the substrate was bound by Arg(265) and Arg(307) . The catalytic residue, Tyr(250) , is under the hydrogen bond network to facilitate its deprotonation acting as a general base, removing an acetate group of secondary amines as glyoxylate. Thus, IdaA is a secondary amine oxidase, and our findings improve understanding of molecular mechanism involved in the bioremediation of EDTA and the metabolism of secondary amines.

Structural and Biochemical Characterization of Iminodiacetate Oxidase from Chelativorans sp. BNC1.,Kang C, Jun SY, Bravo AG, Vargas EM, Liu H, Lewis KM, Xun L Mol Microbiol. 2019 Oct 3. doi: 10.1111/mmi.14399. PMID:31580513<ref>PMID:31580513</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6pxs" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Chelativorans sp. BNC1]]
[[Category: Large Structures]]
[[Category: Jun SY]]
[[Category: Kang C]]
[[Category: Lewis KM]]
[[Category: Xun L]]