View source for 6qd3


==MloK1 model from single particle analysis of 2D crystals, class 7 (intermediate conformation)==
<SX load='6qd3' size='340' side='right' viewer='molstar' caption='[[6qd3]], [[Resolution|resolution]] 5.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6qd3]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QD3 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6QD3 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6i9d|6i9d]], [[6iax|6iax]], [[6qcy|6qcy]], [[6qcz|6qcz]], [[6qd0|6qd0]], [[6qd1|6qd1]], [[6qd2|6qd2]], [[6qd4|6qd4]]</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6qd3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qd3 OCA], [http://pdbe.org/6qd3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6qd3 RCSB], [http://www.ebi.ac.uk/pdbsum/6qd3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6qd3 ProSAT]</span></td></tr>
</table>
== Function ==
[[http://www.uniprot.org/uniprot/CNGK1_RHILO CNGK1_RHILO]] Cyclic nucleotide-regulated potassium channel activated by cAMP.<ref>PMID:15550244</ref>  
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== Publication Abstract from PubMed ==
Electron crystallography can reveal the structure of membrane proteins within 2D crystals under close-to-native conditions. High-resolution structural information can only be reached if crystals are perfectly flat and highly ordered. In practice, such crystals are difficult to obtain. Available image unbending algorithms correct for disorder, but only perform well on images of non-tilted, flat crystals, while out-of-plane distortions are not addressed. Here, we present an approach that employs single-particle refinement procedures to locally unbend crystals in 3D. With this method, density maps of the MloK1 potassium channel with a resolution of 4 A were obtained from images of 2D crystals that do not diffract beyond 10 A. Furthermore, 3D classification allowed multiple structures to be resolved, revealing a series of MloK1 conformations within a single 2D crystal. This conformational heterogeneity explains the poor diffraction observed and is related to channel function. The approach is implemented in the FOCUS package.

Retrieving high-resolution information from disordered 2D crystals by single-particle cryo-EM.,Righetto RD, Biyani N, Kowal J, Chami M, Stahlberg H Nat Commun. 2019 Apr 12;10(1):1722. doi: 10.1038/s41467-019-09661-5. PMID:30979902<ref>PMID:30979902</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 6qd3" style="background-color:#fffaf0;"></div>
== References ==
<references/>
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[[Category: Large Structures]]
[[Category: Biyani, N]]
[[Category: Chami, M]]
[[Category: Kowal, J]]
[[Category: Righetto, R]]
[[Category: Stahlberg, H]]
[[Category: Cyclic nucleotide-binding domain]]
[[Category: Ion channel]]
[[Category: Ion transport]]
[[Category: Membrane protein]]
[[Category: Voltage-gated potassium channel]]