View source for 6qf5


==X-Ray structure of human Aquaporin 2 crystallized on a silicon chip==
<StructureSection load='6qf5' size='340' side='right'caption='[[6qf5]], [[Resolution|resolution]] 3.70&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6qf5]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QF5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6QF5 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.7&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CD:CADMIUM+ION'>CD</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6qf5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qf5 OCA], [https://pdbe.org/6qf5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6qf5 RCSB], [https://www.ebi.ac.uk/pdbsum/6qf5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6qf5 ProSAT]</span></td></tr>
</table>
== Disease ==
[https://www.uniprot.org/uniprot/AQP2_HUMAN AQP2_HUMAN] Nephrogenic diabetes insipidus. The disease is caused by mutations affecting the gene represented in this entry.
== Function ==
[https://www.uniprot.org/uniprot/AQP2_HUMAN AQP2_HUMAN] Forms a water-specific channel that provides the plasma membranes of renal collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Efficient and reliable sample delivery has remained one of the bottlenecks for serial crystallography experiments. Compared with other methods, fixed-target sample delivery offers the advantage of significantly reduced sample consumption and shorter data collection times owing to higher hit rates. Here, a new method of on-chip crystallization is reported which allows the efficient and reproducible growth of large numbers of protein crystals directly on micro-patterned silicon chips for in-situ serial crystallography experiments. Crystals are grown by sitting-drop vapor diffusion and previously established crystallization conditions can be directly applied. By reducing the number of crystal-handling steps, the method is particularly well suited for sensitive crystal systems. Excessive mother liquor can be efficiently removed from the crystals by blotting, and no sealing of the fixed-target sample holders is required to prevent the crystals from dehydrating. As a consequence, 'naked' crystals are obtained on the chip, resulting in very low background scattering levels and making the crystals highly accessible for external manipulation such as the application of ligand solutions. Serial diffraction experiments carried out at cryogenic temperatures at a synchrotron and at room temperature at an X-ray free-electron laser yielded high-quality X-ray structures of the human membrane protein aquaporin 2 and two new ligand-bound structures of thermolysin and the human kinase DRAK2. The results highlight the applicability of the method for future high-throughput on-chip screening of pharmaceutical compounds.

On-chip crystallization for serial crystallography experiments and on-chip ligand-binding studies.,Lieske J, Cerv M, Kreida S, Komadina D, Fischer J, Barthelmess M, Fischer P, Pakendorf T, Yefanov O, Mariani V, Seine T, Ross BH, Crosas E, Lorbeer O, Burkhardt A, Lane TJ, Guenther S, Bergtholdt J, Schoen S, Tornroth-Horsefield S, Chapman HN, Meents A IUCrJ. 2019 Jun 19;6(Pt 4):714-728. doi: 10.1107/S2052252519007395. eCollection, 2019 Jul 1. PMID:31316815<ref>PMID:31316815</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6qf5" style="background-color:#fffaf0;"></div>

==See Also==
*[[Aquaporin 3D structures|Aquaporin 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Homo sapiens]]
[[Category: Large Structures]]
[[Category: Barthelmess M]]
[[Category: Bergtholdt J]]
[[Category: Burkhardt A]]
[[Category: Cerv M]]
[[Category: Chapman HN]]
[[Category: Crosas E]]
[[Category: Fischer P]]
[[Category: Guenther S]]
[[Category: Kreida S]]
[[Category: Lane TJ]]
[[Category: Lieske J]]
[[Category: Lorbeer O]]
[[Category: Mariani V]]
[[Category: Meents A]]
[[Category: Pakendorf T]]
[[Category: Ross BH]]
[[Category: Schoen S]]
[[Category: Seine T]]
[[Category: Tornroth-Horsefield S]]
[[Category: Yefanov O]]