View source for 6qsk


==Crystal structure of a nucleotide sugar transporter with bound nucleotide monophosphate.==
<StructureSection load='6qsk' size='340' side='right'caption='[[6qsk]], [[Resolution|resolution]] 3.39&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6qsk]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QSK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6QSK FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.394&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=5GP:GUANOSINE-5-MONOPHOSPHATE'>5GP</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=OLC:(2R)-2,3-DIHYDROXYPROPYL+(9Z)-OCTADEC-9-ENOATE'>OLC</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6qsk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qsk OCA], [https://pdbe.org/6qsk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6qsk RCSB], [https://www.ebi.ac.uk/pdbsum/6qsk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6qsk ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/GMT1_YEAST GMT1_YEAST] Involved in the import of GDP-mannose from the cytoplasm into the Golgi lumen. Defective copy causes severe glycosylation defect and abnormal retention of soluble endoplasmic reticulum proteins. Involved in vanadate sensitivity.<ref>PMID:10570930</ref> <ref>PMID:11067855</ref> <ref>PMID:12478588</ref> <ref>PMID:15494368</ref> <ref>PMID:2014241</ref> <ref>PMID:2137555</ref> <ref>PMID:7672592</ref> <ref>PMID:7877969</ref> <ref>PMID:8632002</ref> <ref>PMID:9184829</ref> <ref>PMID:9335583</ref> <ref>PMID:9395539</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Nucleotide sugars are the activated form of monosaccharides used by glycosyltransferases during glycosylation. In eukaryotes the SLC35 family of solute carriers are responsible for their selective uptake into the Endoplasmic Reticulum or Golgi apparatus. The structure of the yeast GDP-mannose transporter, Vrg4, revealed a requirement for short chain lipids and a marked difference in transport rate between the nucleotide sugar and nucleoside monophosphate, suggesting a complex network of regulatory elements control transport into these organelles. Here we report the crystal structure of the GMP bound complex of Vrg4, revealing the molecular basis for GMP recognition and transport. Molecular dynamics, combined with biochemical analysis, reveal a lipid mediated dimer interface and mechanism for coordinating structural rearrangements during transport. Together these results provide further insight into how SLC35 family transporters function within the secretory pathway and sheds light onto the role that membrane lipids play in regulating transport across the membrane.

Structural basis for substrate specificity and regulation of nucleotide sugar transporters in the lipid bilayer.,Parker JL, Corey RA, Stansfeld PJ, Newstead S Nat Commun. 2019 Oct 11;10(1):4657. doi: 10.1038/s41467-019-12673-w. PMID:31604945<ref>PMID:31604945</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6qsk" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae S288C]]
[[Category: Newstead S]]
[[Category: Parker JL]]