View source for 6qv0


==Structure of ATP-bound outward-facing TM287/288 in complex with sybody Sb_TM35==
<StructureSection load='6qv0' size='340' side='right'caption='[[6qv0]], [[Resolution|resolution]] 3.12&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6qv0]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermotoga_maritima_MSB8 Thermotoga maritima MSB8] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QV0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6QV0 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.12&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6qv0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qv0 OCA], [https://pdbe.org/6qv0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6qv0 RCSB], [https://www.ebi.ac.uk/pdbsum/6qv0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6qv0 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q9WYC3_THEMA Q9WYC3_THEMA] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
ABC exporters harness the energy of ATP to pump substrates across membranes. Extracellular gate opening and closure are key steps of the transport cycle, but the underlying mechanism is poorly understood. Here, we generated a synthetic single domain antibody (sybody) that recognizes the heterodimeric ABC exporter TM287/288 exclusively in the presence of ATP, which was essential to solve a 3.2 A crystal structure of the outward-facing transporter. The sybody binds to an extracellular wing and strongly inhibits ATPase activity by shifting the transporter's conformational equilibrium towards the outward-facing state, as shown by double electron-electron resonance (DEER). Mutations that facilitate extracellular gate opening result in a comparable equilibrium shift and strongly reduce ATPase activity and drug transport. Using the sybody as conformational probe, we demonstrate that efficient extracellular gate closure is required to dissociate the NBD dimer after ATP hydrolysis to reset the transporter back to its inward-facing state.

The extracellular gate shapes the energy profile of an ABC exporter.,Hutter CAJ, Timachi MH, Hurlimann LM, Zimmermann I, Egloff P, Goddeke H, Kucher S, Stefanic S, Karttunen M, Schafer LV, Bordignon E, Seeger MA Nat Commun. 2019 May 21;10(1):2260. doi: 10.1038/s41467-019-09892-6. PMID:31113958<ref>PMID:31113958</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6qv0" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Synthetic construct]]
[[Category: Thermotoga maritima MSB8]]
[[Category: Egloff P]]
[[Category: Huerlimann LM]]
[[Category: Hutter CAJ]]
[[Category: Seeger MA]]
[[Category: Zimmermann I]]