View source for 6r86


==Yeast Vms1-60S ribosomal subunit complex (post-state)==
<SX load='6r86' size='340' side='right' viewer='molstar' caption='[[6r86]], [[Resolution|resolution]] 3.40&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6r86]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6R86 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6R86 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.4&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6r86 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6r86 OCA], [https://pdbe.org/6r86 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6r86 RCSB], [https://www.ebi.ac.uk/pdbsum/6r86 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6r86 ProSAT]</span></td></tr>
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== Function ==
[https://www.uniprot.org/uniprot/RL13A_YEAST RL13A_YEAST] 
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== Publication Abstract from PubMed ==
Ribosome-associated quality control (RQC) provides a rescue pathway for eukaryotic cells to process faulty proteins after translational stalling of cytoplasmic ribosomes(1-6). After dissociation of ribosomes, the stalled tRNA-bound peptide remains associated with the 60S subunit and extended by Rqc2 by addition of C-terminal alanyl and threonyl residues (CAT tails)(7-9), whereas Vms1 catalyses cleavage and release of the peptidyl-tRNA before or after addition of CAT tails(10-12). In doing so, Vms1 counteracts CAT-tailing of nuclear-encoded mitochondrial proteins that otherwise drive aggregation and compromise mitochondrial and cellular homeostasis(13). Here we present structural and functional insights into the interaction of Saccharomyces cerevisiae Vms1 with 60S subunits in pre- and post-peptidyl-tRNA cleavage states. Vms1 binds to 60S subunits with its Vms1-like release factor 1 (VLRF1), zinc finger and ankyrin domains. VLRF1 overlaps with the Rqc2 A-tRNA position and interacts with the ribosomal A-site, projecting its catalytic GSQ motif towards the CCA end of the tRNA, its Y285 residue dislodging the tRNA A73 for nucleolytic cleavage. Moreover, in the pre-state, we found the ABCF-type ATPase Arb1 in the ribosomal E-site, which stabilizes the delocalized A73 of the peptidyl-tRNA and stimulates Vms1-dependent tRNA cleavage. Our structural analysis provides mechanistic insights into the interplay of the RQC factors Vms1, Rqc2 and Arb1 and their role in the protection of mitochondria from the aggregation of toxic proteins.

Structure and function of Vms1 and Arb1 in RQC and mitochondrial proteome homeostasis.,Su T, Izawa T, Thoms M, Yamashita Y, Cheng J, Berninghausen O, Hartl FU, Inada T, Neupert W, Beckmann R Nature. 2019 Jun 12. pii: 10.1038/s41586-019-1307-z. doi:, 10.1038/s41586-019-1307-z. PMID:31189955<ref>PMID:31189955</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 6r86" style="background-color:#fffaf0;"></div>

==See Also==
*[[Ribosome 3D structures|Ribosome 3D structures]]
== References ==
<references/>
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</SX>
[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Beckmann R]]
[[Category: Berninghausen O]]
[[Category: Cheng J]]
[[Category: Inada T]]
[[Category: Izawa T]]
[[Category: Neupert W]]
[[Category: Su T]]
[[Category: Yamashita Y]]