View source for 6rgu


==Photorhabdus asymbiotica lectin PHL in complex with 3-O-methyl-D-glucose==
<StructureSection load='6rgu' size='340' side='right'caption='[[6rgu]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6rgu]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Photorhabdus_asymbiotica Photorhabdus asymbiotica]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6RGU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6RGU FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.75&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=3MG:3-O-METHYL-BETA-D-GLUCOPYRANOSE'>3MG</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6rgu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6rgu OCA], [https://pdbe.org/6rgu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6rgu RCSB], [https://www.ebi.ac.uk/pdbsum/6rgu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6rgu ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/C7BLE4_PHOAA C7BLE4_PHOAA] 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
O-methylation is an unusual sugar modification with a function that is not fully understood. Given its occurrence and recognition by lectins involved in the immune response, methylated sugars were proposed to represent a conserved pathogen-associated molecular pattern. We describe the interaction of O-methylated saccharides with two beta-propeller lectins, the newly described PLL2 from the entomopathogenic bacterium Photorhabdus laumondii, and its homologue PHL from the related human pathogen Photorhabdus asymbiotica. The crystal structures of PLL2 and PHL revealed up to 10 out of 14 potential binding sites per protein subunit to be occupied with O-methylated structures. The avidity effect strengthens the interaction by 4 orders of magnitude. PLL2 and PHL also interfere with the early immune response by modulating the production of reactive oxygen species and phenoloxidase activity. Since bacteria from Photorhabdus spp. have a complex life cycle involving pathogenicity towards different hosts, the involvement of PLL2 and PHL might contribute to the pathogen overcoming insect and human immune system defenses in the early stages of infection.

Heptabladed beta-propeller lectins PLL2 and PHL from Photorhabdus spp. recognize O-methylated sugars and influence the host immune system.,Fujdiarova E, Houser J, Dobes P, Paulikova G, Kondakov N, Kononov L, Hyrsl P, Wimmerova M FEBS J. 2020 Jun 19. doi: 10.1111/febs.15457. PMID:32559333<ref>PMID:32559333</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6rgu" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Photorhabdus asymbiotica]]
[[Category: Fujdiarova E]]
[[Category: Houser J]]
[[Category: Jancarikova G]]
[[Category: Wimmerova M]]