View source for 6rja


==Cryo-EM structure of St1Cas9-sgRNA-tDNA20-AcrIIA6 dimeric assembly.==
<SX load='6rja' size='340' side='right' viewer='molstar' caption='[[6rja]], [[Resolution|resolution]] 3.00&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6rja]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptococcus_phage_D1811 Streptococcus phage D1811], [https://en.wikipedia.org/wiki/Streptococcus_thermophilus Streptococcus thermophilus], [https://en.wikipedia.org/wiki/Streptococcus_thermophilus_LMD-9 Streptococcus thermophilus LMD-9] and [https://en.wikipedia.org/wiki/Streptococcus_virus_2972 Streptococcus virus 2972]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6RJA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6RJA FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6rja FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6rja OCA], [https://pdbe.org/6rja PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6rja RCSB], [https://www.ebi.ac.uk/pdbsum/6rja PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6rja ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A2U7VKE8_9CAUD A0A2U7VKE8_9CAUD] 
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== Publication Abstract from PubMed ==
In the arms race against bacteria, bacteriophages have evolved diverse anti-CRISPR proteins (Acrs) that block CRISPR-Cas immunity. Acrs play key roles in the molecular coevolution of bacteria with their predators, use a variety of mechanisms of action, and provide tools to regulate Cas-based genome manipulation. Here, we present structural and functional analyses of AcrIIA6, an Acr from virulent phages, exploring its unique anti-CRISPR action. Our cryo-EM structures and functional data of AcrIIA6 binding to Streptococcus thermophilus Cas9 (St1Cas9) show that AcrIIA6 acts as an allosteric inhibitor and induces St1Cas9 dimerization. AcrIIA6 reduces St1Cas9 binding affinity for DNA and prevents DNA binding within cells. The PAM and AcrIIA6 recognition sites are structurally close and allosterically linked. Mechanistically, AcrIIA6 affects the St1Cas9 conformational dynamics associated with PAM binding. Finally, we identify a natural St1Cas9 variant resistant to AcrIIA6 illustrating Acr-driven mutational escape and molecular diversification of Cas9 proteins.

Cas9 Allosteric Inhibition by the Anti-CRISPR Protein AcrIIA6.,Fuchsbauer O, Swuec P, Zimberger C, Amigues B, Levesque S, Agudelo D, Duringer A, Chaves-Sanjuan A, Spinelli S, Rousseau GM, Velimirovic M, Bolognesi M, Roussel A, Cambillau C, Moineau S, Doyon Y, Goulet A Mol Cell. 2019 Sep 26. pii: S1097-2765(19)30697-5. doi:, 10.1016/j.molcel.2019.09.012. PMID:31604602<ref>PMID:31604602</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 6rja" style="background-color:#fffaf0;"></div>

==See Also==
*[[Endonuclease 3D structures|Endonuclease 3D structures]]
== References ==
<references/>
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</SX>
[[Category: Large Structures]]
[[Category: Streptococcus phage D1811]]
[[Category: Streptococcus thermophilus]]
[[Category: Streptococcus thermophilus LMD-9]]
[[Category: Streptococcus virus 2972]]
[[Category: Cambillau C]]
[[Category: Chaves-Sanjuan A]]
[[Category: Goulet A]]