View source for 6roj


==Cryo-EM structure of the activated Drs2p-Cdc50p==
<SX load='6roj' size='340' side='right' viewer='molstar' caption='[[6roj]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6roj]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Klebsiella_pneumoniae Klebsiella pneumoniae] and [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ROJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6ROJ FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=2Y5:(2R)-1-{[(R)-HYDROXY{[(1R,2R,3R,4R,5S,6R)-2,3,5,6-TETRAHYDROXY-4-(PHOSPHONOOXY)CYCLOHEXYL]OXY}PHOSPHORYL]OXY}-3-(OCTADECANOYLOXY)PROPAN-2-YL+(5Z,8Z,11Z,14Z)-ICOSA-5,8,11,14-TETRAENOATE'>2Y5</scene>, <scene name='pdbligand=BFD:ASPARTATE+BERYLLIUM+TRIFLUORIDE'>BFD</scene>, <scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6roj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6roj OCA], [https://pdbe.org/6roj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6roj RCSB], [https://www.ebi.ac.uk/pdbsum/6roj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6roj ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/DCOA_KLEPN DCOA_KLEPN] Catalyzes the decarboxylation of oxaloacetate coupled to Na(+) translocation.[https://www.uniprot.org/uniprot/ATC3_YEAST ATC3_YEAST] This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of phospholipids (Potential). Seems to be involved in ribosome assembly.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Type 4 P-type ATPases (P4-ATPases) are lipid flippases that drive the active transport of phospholipids from exoplasmic or luminal leaflets to cytosolic leaflets of eukaryotic membranes. The molecular architecture of P4-ATPases and the mechanism through which they recognize and transport lipids have remained unknown. Here we describe the cryo-electron microscopy structure of the P4-ATPase Drs2p-Cdc50p, a Saccharomyces cerevisiae lipid flippase that is specific to phosphatidylserine and phosphatidylethanolamine. Drs2p-Cdc50p is autoinhibited by the C-terminal tail of Drs2p, and activated by the lipid phosphatidylinositol-4-phosphate (PtdIns4P or PI4P). We present three structures that represent the complex in an autoinhibited, an intermediate and a fully activated state. The analysis highlights specific features of P4-ATPases and reveals sites of autoinhibition and PI4P-dependent activation. We also observe a putative lipid translocation pathway in this flippase that involves a conserved PISL motif in transmembrane segment 4 and polar residues of transmembrane segments 2 and 5, in particular Lys1018, in the centre of the lipid bilayer.

Structure and autoregulation of a P4-ATPase lipid flippase.,Timcenko M, Lyons JA, Januliene D, Ulstrup JJ, Dieudonne T, Montigny C, Ash MR, Karlsen JL, Boesen T, Kuhlbrandt W, Lenoir G, Moeller A, Nissen P Nature. 2019 Jun 26. pii: 10.1038/s41586-019-1344-7. doi:, 10.1038/s41586-019-1344-7. PMID:31243363<ref>PMID:31243363</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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<div class="pdbe-citations 6roj" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</SX>
[[Category: Klebsiella pneumoniae]]
[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae S288C]]
[[Category: Ash MR]]
[[Category: Boesen T]]
[[Category: Dieudonne T]]
[[Category: Januliene D]]
[[Category: Karlsen JL]]
[[Category: Kuhlbrandt W]]
[[Category: Lenoir G]]
[[Category: Lyons JA]]
[[Category: Moeller A]]
[[Category: Montigny C]]
[[Category: Nissen P]]
[[Category: Timcenko M]]
[[Category: Ulstrup JJ]]