View source for 6rt8


==Structure of catharanthine synthase - an alpha-beta hydrolase from Catharanthus roseus with a cleaviminium intermediate bound==
<StructureSection load='6rt8' size='340' side='right'caption='[[6rt8]], [[Resolution|resolution]] 2.19&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6rt8]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Catharanthus_roseus Catharanthus roseus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6RT8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6RT8 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.19&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=KJE:18-carboxymethoxy-cleaviminium'>KJE</scene>, <scene name='pdbligand=P6G:HEXAETHYLENE+GLYCOL'>P6G</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6rt8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6rt8 OCA], [https://pdbe.org/6rt8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6rt8 RCSB], [https://www.ebi.ac.uk/pdbsum/6rt8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6rt8 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/CS_CATRO CS_CATRO] Component of iboga and aspidosperma monoterpenoid indole alkaloids (MIAs, e.g. tabersonine and catharanthine) biosynthesis pathway from 19E-geissoschizine. Catalyzes the conversion of O-acetylstemmadenine (OAS) to catharanthine.<ref>PMID:29511102</ref> <ref>PMID:29724909</ref> 
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Cycloaddition reactions generate chemical complexity in a single step. Here we report the crystal structures of three homologous plant-derived cyclases involved in the biosynthesis of iboga and aspidosperma alkaloids. These enzymes act on the same substrate, named angryline, to generate three distinct scaffolds. Mutational analysis reveals how these highly similar enzymes control regio- and stereo-selectivity.

Structural basis of cycloaddition in biosynthesis of iboga and aspidosperma alkaloids.,Caputi L, Franke J, Bussey K, Farrow SC, Vieira IJC, Stevenson CEM, Lawson DM, O'Connor SE Nat Chem Biol. 2020 Feb 17. pii: 10.1038/s41589-019-0460-x. doi:, 10.1038/s41589-019-0460-x. PMID:32066966<ref>PMID:32066966</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6rt8" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Catharanthus roseus]]
[[Category: Large Structures]]
[[Category: Bussey K]]
[[Category: Caputi L]]
[[Category: Curcino Vieira IJ]]
[[Category: Farrow SC]]
[[Category: Franke J]]
[[Category: Lawson DM]]
[[Category: O'Connor SE]]
[[Category: Stevenson CEM]]