View source for 6rtv


==Crystal Structure of Glucuronoyl Esterase from Cerrena unicolor inactive S270A variant==
<StructureSection load='6rtv' size='340' side='right'caption='[[6rtv]], [[Resolution|resolution]] 1.46&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6rtv]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Cerrena_unicolor Cerrena unicolor]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6RTV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6RTV FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.46&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6rtv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6rtv OCA], [https://pdbe.org/6rtv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6rtv RCSB], [https://www.ebi.ac.uk/pdbsum/6rtv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6rtv ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/GCE_CERUI GCE_CERUI] Glucuronoyl esterase which may play a significant role in biomass degradation, as it is considered to disconnect hemicellulose from lignin through the hydrolysis of the ester bond between 4-O-methyl-D-glucuronic acid residues of glucuronoxylans and aromatic alcohols of lignin (PubMed:25425346, PubMed:26712478).<ref>PMID:25425346</ref> <ref>PMID:26712478</ref> 
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== Publication Abstract from PubMed ==
Structural and functional studies were conducted of the glucuronoyl esterase (GE) from Cerrena unicolor (CuGE), an enzyme catalyzing cleavage of lignin-carbohydrate ester bonds. CuGE is an alpha/beta-hydrolase belonging to carbohydrate esterase family 15 (CE15). The enzyme is modular, comprised of a catalytic and a carbohydrate-binding domain. SAXS data show CuGE as an elongated rigid molecule where the two domains are connected by a rigid linker. Detailed structural information of the catalytic domain in its apo- and inactivated form and complexes with aldouronic acids reveal well-defined binding of the 4-O-methyl-a-D-glucuronoyl moiety, not influenced by the nature of the attached xylo-oligosaccharide. Structural and sequence comparisons within CE15 enzymes reveal two distinct structural subgroups. CuGE belongs to the group of fungal CE15-B enzymes with an open and flat substrate-binding site. The interactions between CuGE and its natural substrates are explained and rationalized by the structural results, microscale thermophoresis and isothermal calorimetry.

The structural basis of fungal glucuronoyl esterase activity on natural substrates.,Ernst HA, Mosbech C, Langkilde AE, Westh P, Meyer AS, Agger JW, Larsen S Nat Commun. 2020 Feb 24;11(1):1026. doi: 10.1038/s41467-020-14833-9. PMID:32094331<ref>PMID:32094331</ref>

From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6rtv" style="background-color:#fffaf0;"></div>

==See Also==
*[[Methylesterase 3D structures|Methylesterase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Cerrena unicolor]]
[[Category: Large Structures]]
[[Category: Agger JW]]
[[Category: Ernst HA]]
[[Category: Langkilde A]]
[[Category: Larsen S]]
[[Category: Meyer A]]
[[Category: Mosbech C]]
[[Category: Westh P]]